Unusual behavior exhibited by multistranded guanine-rich DNA complexes

Biopolymers
K Poon, R B Macgregor

Abstract

The structural properties of oligonucleotides containing two different types of G-rich sequences at the 3'-ends were compared. It is shown that oligonucleotides with uninterrupted runs of guanine residues at the 3'-end, e.g., d(T15G12), form multistranded structures stabilized by guanine-guanine interactions. The chemical and physical properties of these complexes differ from those of the complexes formed by oligonucleotides with telomere-like sequences, e.g., d(T15G4T2G4). In methylation protection and methylation interference experiments, we found all the guanines in complexes formed by d(T15G15) and d(T15G12) to be accessible to methylation. Furthermore, the methylated monomers retain the ability to polymerize. This contrasts with the inaccessibility of the guanines in d(T15G4T2G4) to methylation and the inability of the methylated monomer to form supramolecular structures. The stoichiometry of the complexes arising from the two types of oligonucleotides also differs. The complexes formed by d(T15G15) consist of consecutive integer numbers of DNA strands, whereas complexes formed by telomere-like oligonucleotides contain 1, 2, 4, or multiples of four strands. Magnesium ions favor formation of high molecular weight complexes ...Continue Reading

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Citations

Sep 10, 2013·Nucleic Acids Research·Christina L WysoczynskiChristopher S Malarkey
Aug 14, 2012·Nature Methods·Jerrod J SchwartzJay Shendure
Feb 4, 2010·Biophysical Chemistry·Rashid M Abu-GhazalahRobert B Macgregor
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Oct 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sulayman A Oladepo, Basiru O Yusuf
Apr 10, 2021·Nucleic Acids Research·Chengtao XuHong Liu
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