Unusual diversity of acquired β-lactamases in multidrug-resistant Pseudomonas aeruginosa isolates in a Mexican hospital.

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
J Castillo-VeraGerardo Aparicio-Ozores

Abstract

To investigate the presence of extended spectrum and metallo β-lactamases (MBLs) in Pseudomonas aeruginosa isolates which are resistant to imipenem and ceftazidime that were isolated in a hospital in Mexico. Pulsed-field gel electrophoresis (PFGE) revealed the presence of four clonal types among the 14 isolates. All these genes were found either alone or simultaneously in the P. aeruginosa strains in the following five different arrangements: <bla(GES-5)>; <bla(GES-5), bla(VIM-11)>; <bla(GES-5), bla(VIM-2), bla(VIM-11)>; <bla(GES-5), bla(OXA-2)>; and <bla(GES-5), bla(VIM-2), bla(VIM-11), and bla(OXA-2)>. Class 1 integrons were detected and contained the cassettes bla(GES-5) and bla(OXA-2), but not that of bla(VIM). bla(VIM) genes occurred only in the chromosome, while bla(GES-5) was located in the chromosome and in the plasmids. To our knowledge, this is the first description of P. aeruginosa strains simultaneously producing the VIM-2 and VIM-11 variants, and the combination of GES-5 and MBL carbapenemases, which determines a major challenge for the clinical microbiology laboratory and a remarkable epidemiological risk for the nosocomial spread of multidrug-resistant determinants.

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Citations

Jul 25, 2013·Expert Review of Anti-infective Therapy·Juan J MayaMaria V Villegas
Aug 13, 2015·Journal of Immunoassay & Immunochemistry·Paul Akinniyi AkindutiOluwaseun Ejilude
Apr 22, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G MeletisN Malisiovas
Dec 6, 2016·Expert Review of Anti-infective Therapy·Kevin Escandón-VargasMaría Virginia Villegas
Jan 4, 2019·Journal of Computer-aided Molecular Design·Francesca SpyrakisDonatella Tondi
Aug 20, 2020·Expert Review of Anti-infective Therapy·Juan Carlos García-BetancurMaría Virginia Villegas
May 19, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·P Akinniyi AkindutiOluwadun Afolabi

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Datasets Mentioned

BETA
GQ242167
AM988778
FJ752628
GQ202693
DQ899757

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

- UPGMA
UPGMA
Dendro

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