PMID: 9541559Apr 29, 1998Paper

Unusual gene arrangement of the bidirectional hydrogenase and functional analysis of its diaphorase subunit HoxU in respiration of the unicellular cyanobacterium anacystis nidulans

Current Microbiology
Gudrun BoisonHermann Bothe

Abstract

The bidirectional, NAD+-dependent hydrogenase from cyanobacteria is encoded by the structural genes hoxFUYH, which have been found to be clustered, though interspersed with different open reading frames (ORFs), in the heterocystous, N2-fixing Anabaena variabilis and in the unicellular Synechocystis PCC 6803. In another unicellular, non N2-fixing cyanobacterium, Anacystis nidulans, hoxF has now been identified as being separated by at least 16 kb from the residual structural genes hoxUYH. An ORF (termed hoxE gene) is located immediately upstream of hoxF in A. nidulans and in Synechocystis. Its deduced amino acid sequence shows similarities to the NuoE subunit of NADH dehydrogenase I of E. coli, to the homologous subunit of respiratory complex I in mitochondria, and also to the first 104 amino acids of HoxF in A. nidulans and Synechocystis. The diversity in the arrangement of hydrogenase genes in cyanobacteria is puzzling. The subunits HoxE, HoxF, and HoxU of the diaphorase part of the bidirectional hydrogenase have been discussed to be shared both by respiratory complex I and bidirectional hydrogenase in cyanobacteria. Different hoxU mutants were obtained by inserting a lacZKmR cassette into the gene both in A. nidulans and Anac...Continue Reading

Citations

May 30, 2002·Biochimica Et Biophysica Acta·Oliver SchmitzThomas Happe
Feb 28, 2003·European Journal of Biochemistry·Markus LaukelJulia A Vorholt
May 27, 2015·International Journal of Molecular Sciences·Namita Khanna, Peter Lindblad
Feb 7, 2008·Physiologia Plantarum·Natalia Battchikova, Eva-Mari Aro
Oct 2, 2007·FEMS Microbiology Reviews·Paula TamagniniPeter Lindblad
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Hermann BotheWilliam E Newton
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Paula TamagniniPeter Lindblad
Jul 17, 2007·Applied and Environmental Microbiology·Johannes SjöholmPeter Lindblad
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer

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