Unusual nucleotide-binding properties of the chloroplast protein import receptor, atToc33

FEBS Letters
Henrik AronssonPaul Jarvis

Abstract

Arabidopsis Toc33 (atToc33) is a GTP-binding protein of the chloroplast outer envelope membrane. We studied its nucleotide-binding properties in vitro, and found that it binds GTP, GDP and XTP, with similar efficiencies, but not ATP. We further demonstrated that atToc33 has intrinsic GTPase activity. Mutations within the putative G4 motif of the atToc33 nucleotide-binding domain (D217N, D219N and E220Q) had no effect on nucleotide specificity or GTPase activity. Similarly, a mutation in the newly assigned G5 motif (E208Q) did not affect nucleotide specificity or GTPase activity. Furthermore, the D217N and D219N mutations did not affect atToc33 functionality in vivo. The data demonstrate that atToc33 belongs to a novel class of GTPases with unusual nucleotide-binding properties.

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Citations

Dec 11, 2007·Trends in Cell Biology·Mislav OrebEnrico Schleiff
Mar 23, 2007·The Plant Journal : for Cell and Molecular Biology·Sabina KovachevaPaul Jarvis
May 10, 2008·The Plant Journal : for Cell and Molecular Biology·Nicolas FourrierGlenn Thorlby
Jul 28, 2007·The Plant Journal : for Cell and Molecular Biology·Henrik AronssonPaul Jarvis
Oct 16, 2012·Biochimica Et Biophysica Acta·Lan-Xin Shi, Steven M Theg
Feb 6, 2017·International Journal of Molecular Sciences·Congfei YinHenrik Aronsson
Aug 10, 2005·Journal of Experimental Botany·Jocelyn Bédard, Paul Jarvis
Jun 11, 2008·The Journal of Biological Chemistry·Patrick KoenigIvo Tews
Jan 31, 2007·The Journal of Biological Chemistry·L Evan ReddickBarry D Bruce
Mar 6, 2007·The Journal of Biological Chemistry·Yi-Hung YehChwan-Deng Hsiao

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