Unusual pattern of ribonucleic acid components in the ribosome of Crithidia fasciculata, a trypanosomatid protozoan.

Molecular and Cellular Biology
M W Gray

Abstract

In a previous study from this laboratory, presumptive ribosomal ribonucleic acid (RNA) species were identified in the total cellular RNA directly extracted from intact cells of the trypanosomatid protozoan Crithidia fasciculata (M. W. Gray, Can. J. Biochem. 57:914-926, 1979). The results suggested that the C. fasciculata ribosome might be unusual in containing three novel, low-molecular-weight ribosomal RNA components, designated e, f, and g (apparent chain lengths 240, 195, and 135 nucleotides, respectively), in addition to analogs of eucaryotic 5S (species h) and 5.8S (species i) ribosomal RNAs. In the present study, all of the presumptive ribosomal RNAs were indeed found to be associated with purified C. fasciculata ribosomes, and their localization was investigated in subunits produced under different conditions of ribosome dissociation. When ribosomes were dissociated in a high-potassium (880 mM K+, 12.5 mM Mg2+) medium, species e to i were all found in the large ribosomal subunit, which also contained an additional, transfer RNA-sized component (species j). However, when subunits were prepared in a low-magnesium (60 mM K+, 0.1 mM Mg2+) medium, two of the novel species (e and g) did not remain with the large subunit, but w...Continue Reading

References

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Citations

Aug 2, 2003·Biochemical and Biophysical Research Communications·Ivor TittawellaVladimir Baranov
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zheng LiuJoachim Frank
Feb 14, 1992·Biochemical and Biophysical Research Communications·D A Campbell
Apr 4, 2000·FEBS Letters·I Tittawella, V Baranov
Apr 1, 1992·FEMS Microbiology Letters·R Hernández-RivasR Hernández
May 17, 2019·Biological Reviews of the Cambridge Philosophical Society·Matej VestegJuraj Krajčovič
Feb 28, 2014·RNA Biology·Michael W Gray
Jan 19, 2020·The Journal of Biological Chemistry·Michael W Gray, Venkat Gopalan
Oct 23, 2020·Nucleic Acids Research·Donna MatzovMoran Shalev-Benami
Sep 5, 1990·Journal of Molecular Biology·M N Schnare, M W Gray

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