Unusually stable abnormal karyotype in a highly aggressive melanoma negative for telomerase activity.

Molecular Cytogenetics
Sarantis GagosSophie Dahoun

Abstract

Malignant melanomas are characterized by increased karyotypic complexity, extended aneuploidy and heteroploidy. We report a melanoma metastasis to the peritoneal cavity with an exceptionally stable, abnormal pseudodiploid karyotype as verified by G-Banding, subtelomeric, centromeric and quantitative Fluorescence in Situ Hybridization (FISH). Interestingly this tumor had no detectable telomerase activity as indicated by the Telomere Repeat Amplification Protocol. Telomeric Flow-FISH and quantitative telomeric FISH on mitotic preparations showed that malignant cells had relatively short telomeres. Microsatellite instability was ruled out by the allelic pattern of two major mononucleotide repeats. Our data suggest that a combination of melanoma specific genomic imbalances were sufficient and enough for this fatal tumor progression, that was not accompanied by genomic instability, telomerase activity, or the engagement of the alternative recombinatorial telomere lengthening pathway.

References

Oct 1, 1976·Science·P C Nowell
Jun 30, 1995·Science·N PapadopoulosK W Kinzler
Jan 1, 1999·Nature·C LengauerB Vogelstein
Feb 27, 1999·Experimental Cell Research·C N SprungJ P Murnane
Jul 17, 1999·British Journal of Cancer·C N ParrisR F Newbold
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·D GisselssonN Mandahl
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·W M Abdel-RahmanP A Edwards
May 14, 2003·Cancer Genetics and Cytogenetics·Alice FabariusPeter H Duesberg
Oct 28, 2003·The American Journal of Pathology·Boris C BastianDaniel Pinkel
Nov 18, 2003·International Journal of Cancer. Journal International Du Cancer·Mattias HöglundDouglas Horsman
Jul 29, 2004·Journal of Clinical Pathology·M R Hussein
Nov 19, 2004·Human Molecular Genetics·Mercè Garcia-BarceloPaul K H Tam
Jan 13, 2005·Genes, Chromosomes & Cancer·Mattias HöglundFelix Mitelman
Mar 4, 2005·The International Journal of Biochemistry & Cell Biology·Sarantis Gagos, Irmgard Irminger-Finger
Sep 17, 2005·Genes & Development·Titia de Lange
Oct 26, 2005·Human Molecular Genetics·Alessandra Muntoni, Roger R Reddel
Aug 22, 2006·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Lucia CarvalhoLydia Ferreira

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Citations

Sep 2, 2010·PloS One·Regina Berretta, Pablo Moscato
Feb 22, 2018·International Journal of Molecular Sciences·Marco De VitisAntonella Sgura
May 13, 2018·Genes·Tiago Bordeira GasparJoão Vinagre

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Methods Mentioned

BETA
PCR
Electrophoresis

Software Mentioned

Cytocell
Isis

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