Unveiling Biosynthesis of the Phytohormone Abscisic Acid in Fungi: Unprecedented Mechanism of Core Scaffold Formation Catalyzed by an Unusual Sesquiterpene Synthase

Journal of the American Chemical Society
Junya TakinoHideaki Oikawa

Abstract

Abscisic acid (ABA) is a well-known phytohormone that regulates abiotic stresses. ABA produced by fungi is also proposed to be a virulence factor of fungal pathogens. Although its biosynthetic pathway in fungi was proposed by a series of feeding experiments, the enzyme catalyzing the reaction from farnesyl diphosphate to α-ionylideneethane remains to be identified. In this work, we identified the novel type of sesquiterpene synthase BcABA3 and its unprecedented three-step reaction mechanism involving two neutral intermediates, β-farnesene and allofarnesene. Database searches showed that BcABA3 has no homology with typical sesquiterpene synthases and that the homologous enzyme genes are found in more than 100 bacteria, suggesting that these enzymes form a new family of sesquiterpene synthases.

References

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Citations

May 22, 2019·Bioscience, Biotechnology, and Biochemistry·Junya TakinoHideaki Oikawa
Nov 19, 2019·Bioscience, Biotechnology, and Biochemistry·Hideaki Oikawa
Dec 19, 2019·Journal of Integrative Plant Biology·Kong ChenYang Zhao
Nov 13, 2020·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hideaki Oikawa
Jun 3, 2021·International Journal of Molecular Sciences·Maria Hrmova, Syed Sarfraz Hussain
Feb 28, 2020·Organic Letters·Xingxing Wei, Yudai Matsuda
Oct 14, 2021·Journal of the American Chemical Society·Xingxing WeiYudai Matsuda

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