Unveiling Mycoplasma hyopneumoniae promoters: sequence definition and genomic distribution.

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
Shana de Souto WeberIrene Silveira Schrank

Abstract

Several Mycoplasma species have had their genome completely sequenced, including four strains of the swine pathogen Mycoplasma hyopneumoniae. Nevertheless, little is known about the nucleotide sequences that control transcriptional initiation in these microorganisms. Therefore, with the objective of investigating the promoter sequences of M. hyopneumoniae, 23 transcriptional start sites (TSSs) of distinct genes were mapped. A pattern that resembles the σ(70) promoter -10 element was found upstream of the TSSs. However, no -35 element was distinguished. Instead, an AT-rich periodic signal was identified. About half of the experimentally defined promoters contained the motif 5'-TRTGn-3', which was identical to the -16 element usually found in Gram-positive bacteria. The defined promoters were utilized to build position-specific scoring matrices in order to scan putative promoters upstream of all coding sequences (CDSs) in the M. hyopneumoniae genome. Two hundred and one signals were found associated with 169 CDSs. Most of these sequences were located within 100 nucleotides of the start codons. This study has shown that the number of promoter-like sequences in the M. hyopneumoniae genome is more frequent than expected by chance, i...Continue Reading

References

Nov 1, 1991·Trends in Biochemical Sciences·J A Goodrich, W R McClure
Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Oct 20, 1995·Science·C M FraserJ C Venter
Apr 1, 1994·Computer Applications in the Biosciences : CABIOS·D A Clark, C J Rawlings
Sep 5, 1997·Science·F R BlattnerY Shao
Feb 11, 1998·Journal of Bacteriology·M M WöstenB A van der Zeijst
Nov 10, 2000·Nucleic Acids Research·J WeinerG F Browning
Sep 6, 2001·Microbiology·H JarmerS Knudsen
Sep 13, 2002·Journal of Molecular Biology·Martin I Voskuil, Glenn H Chambliss
Feb 22, 2003·Journal of Molecular Biology·Lise PetersenAnders Krogh
Jun 26, 2003·Nucleic Acids Research·Jacques van Helden
Aug 9, 2003·Nucleic Acids Research·Jennie E MitchellStephen D Minchin
Dec 4, 2003·Environmental Microbiology·Ildefonso CasesVíctor de Lorenzo
May 4, 2004·Nature Structural & Molecular Biology·Bryce E NickelsAnn Hochschild
May 4, 2004·Nature Structural & Molecular Biology·Konstantin BrodolinHermann Heumann
May 8, 2004·Molecular Microbiology·Gilbert OrsiniAnnie Kolb
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Oct 19, 2004·Journal of Bacteriology·F Chris MinionGregory G Mahairas
Oct 22, 2005·Infection and Immunity·Cary AdamsF Chris Minion
Dec 22, 2005·Infection and Immunity·Melissa L MadsenF Chris Minion
Apr 22, 2006·Biochemical Society Symposium·Nora S Miroslavova, Stephen J W Busby
Jun 1, 2006·Microbiology·Shawn R MacLellanTurlough M Finan
Nov 28, 2007·Research in Microbiology·Brian GrechPeter Timms
May 13, 2008·BMC Bioinformatics·Michael DekhtyarVehary Sakanyan
May 23, 2008·Nucleic Acids Research·Morgane Thomas-ChollierJacques van Helden
Sep 20, 2008·Nature Protocols·Jean-Valery TuratsinzeJacques van Helden
Dec 8, 2009·Science·Marc GüellLuis Serrano
May 1, 2010·Microbiology·Stuart W Gardner, F Chris Minion
Oct 7, 2010·Nucleic Acids Research·Alejandra Medina-RiveraJacques van Helden
Dec 15, 2010·Journal of Bacteriology·Wei LiuShaobo Xiao
Feb 22, 2011·Current Opinion in Microbiology·Charles L Turnbough
Nov 17, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Franciele Maboni SiqueiraIrene Silveira Schrank

❮ Previous
Next ❯

Citations

Jan 19, 2013·Database : the Journal of Biological Databases and Curation·Verena WeissJulio Collado-Vides
Jul 31, 2013·Veterinary Research·Gareth A MaglennonUNKNOWN BRaDP1T consortium
Apr 18, 2015·BMC Genomics·Tiago Ebert FritschIrene Silveira Schrank
Oct 22, 2014·PloS One·Franciele Maboni SiqueiraArnaldo Zaha
Dec 23, 2016·PloS One·Amanda Malvessi CattaniIrene Silveira Schrank
Oct 19, 2017·DNA and Cell Biology·Takamichi NijoShigetou Namba
May 23, 2014·Molecular Biology Reports·Franciele Maboni SiqueiraIrene Silveira Schrank
Oct 26, 2018·BMC Genomics·Tiago Ebert FritschIrene Silveira Schrank
Dec 18, 2020·Molecular Systems Biology·Dominick MatteauSébastien Rodrigue

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCRs
PCR

Software Mentioned

Local
BioEdit
Scan
Matrix
MEME
RSAT
- Analysis
RLM
RACE
- Quality

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved