Unveiling the metabolic potential of two soil-derived microbial consortia selected on wheat straw

Scientific Reports
Diego Javier JiménezJan Dirk van Elsas

Abstract

Based on the premise that plant biomass can be efficiently degraded by mixed microbial cultures and/or enzymes, we here applied a targeted metagenomics-based approach to explore the metabolic potential of two forest soil-derived lignocellulolytic microbial consortia, denoted RWS and TWS (bred on wheat straw). Using the metagenomes of three selected batches of two experimental systems, about 1.2 Gb of sequence was generated. Comparative analyses revealed an overrepresentation of predicted carbohydrate transporters (ABC, TonB and phosphotransferases), two-component sensing systems and β-glucosidases/galactosidases in the two consortia as compared to the forest soil inoculum. Additionally, "profiling" of carbohydrate-active enzymes showed significant enrichments of several genes encoding glycosyl hydrolases of families GH2, GH43, GH92 and GH95. Sequence analyses revealed these to be most strongly affiliated to genes present on the genomes of Sphingobacterium, Bacteroides, Flavobacterium and Pedobacter spp. Assembly of the RWS and TWS metagenomes generated 16,536 and 15,902 contigs of ≥10 Kb, respectively. Thirteen contigs, containing 39 glycosyl hydrolase genes, constitute novel (hemi)cellulose utilization loci with affiliation to...Continue Reading

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Citations

Dec 3, 2015·Biotechnology for Biofuels·Diego Javier JiménezJan Dirk van Elsas
Jul 16, 2016·Applied Microbiology and Biotechnology·Diego Javier JiménezJan Dirk van Elsas
Jul 22, 2016·Critical Reviews in Biotechnology·Joshua J RosnowAaron T Wright
Feb 3, 2018·Microbial Ecology·Pilar Eliana Puentes-Téllez, Joana Falcao Salles
Jul 10, 2019·Microbial Ecology·Pilar Eliana Puentes-Téllez, Joana Falcao Salles
Sep 6, 2018·Nature Communications·Ashish A MalikRobert I Griffiths
May 5, 2017·Frontiers in Microbiology·Leandro N LemosJoão C Setubal
May 26, 2017·Scientific Reports·Anna M AlessiNeil C Bruce
Feb 1, 2021·The Science of the Total Environment·Lei JinLikou Zou
Apr 24, 2021·Frontiers in Microbiology·Judith Kraut-CohenEddie Cytryn
May 1, 2021·Microorganisms·Joao Pedro SaraivaUlisses Nunes da Rocha
Jul 3, 2021·International Journal of Environmental Research and Public Health·Ogechukwu Bose ChukwumaNorli Ismail
Oct 2, 2021·Applied Microbiology and Biotechnology·Yanfang WangJan Dirk van Elsas

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Methods Mentioned

BETA
chips
phosphotransferase

Software Mentioned

RAST
FragGeneScan
CLC Genomics Workbench
MetaGeneMark
PICRUSt
dbCAN
HIT
BLASTP
MG
STAMP

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