PMID: 7019913May 1, 1981Paper

Unwinding of double-stranded DNA helix by dehydration

Proceedings of the National Academy of Sciences of the United States of America
C H LeeT Kakefuda

Abstract

Conformation changes of the double-stranded DNA helix in response to dehydration were investigated by monitoring, by agarose gel electrophoresis, the linking number of covalently closed circular DNA generated by ligation of linear DNA in the presence of different organic solvents or different temperatures. It was found that: (i) The DNA helix unwinds upon addition of certain organic solvents or elevation of temperature. (ii) The conformational change observed under the experimental conditions is a continuous process in response to the organic solvent concentration. (iii) The delta H of unwinding one linking of the DNA helix is constant at approximately 12.2 +/- 0.4 kcal/mol (1 kcal = 4.184 kJ); the corresponding delta S and d(delta S)/dn are 2nkR and 2kR, in which n is the relative equivalent linking number (referred to the state of delta S = 0 for unwinding) of the DNA, R is the gas constant, and k is equal to 1117/number of base pairs. The delta H, delta S, and d(delta S)/dn for unwinding i linkings are i X 12.2 kcal/mol, 2inkR, and 2ikR, respectively. (iv) d(delta S)/dn, like k, is inversely proportional to the number of base pairs in DNA. (v) Double-stranded DNAs of different chain lengths have average delta S = 35 cal/mol....Continue Reading

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