Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels

Physical Chemistry Chemical Physics : PCCP
Keitel Cervantes-SalgueroSatoshi Murata

Abstract

We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm-1) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.

References

May 3, 1996·Science·M TrauI A Aksay
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·R G SosnowskiM J Heller
Dec 24, 1998·Nucleic Acids Research·F N RehmanT C Boles
Aug 19, 2000·Nature·B YurkeJ L Neumann
Mar 20, 2001·Nature Reviews. Molecular Cell Biology·C BustamanteG J Wuite
Mar 18, 2004·Oligonucleotides·Jean-Louis Mergny, Laurent Lacroix
Jul 2, 2004·Analytical Chemistry·Rebecca A Zangmeister, Michael J Tarlov
Mar 23, 2005·Nature Methods·Matthew J LangSteven M Block
Feb 10, 2007·Small·Robert J BarsottiFrancesco Stellacci
Nov 14, 2008·Journal of the American Chemical Society·Sumeet MahajanPhilip N Bartlett
Apr 16, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Sagar M AgnihotriKimiko Makino
Nov 22, 2013·Nature Chemistry·Steven M ChirieleisonXi Chen
Oct 25, 2014·Nature Communications·Yun ZhangKhalid Salaita
Dec 4, 2014·Nature Communications·Wooli BaeTae-Young Yoon
Nov 18, 2015·ACS Nano·Majid MosayebiThomas E Ouldridge
Dec 15, 2015·Journal of the American Chemical Society·Laura E Baltierra-JassoSteven W Magennis
Mar 18, 2016·Nature Communications·Darren YangWesley P Wong
Oct 8, 2016·Soft Matter·Reghan J Hill
Feb 12, 2017·Accounts of Chemical Research·Dong WangDan Luo
Feb 18, 2017·Science·Franziska BleichertJames M Berger

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Citations

Sep 12, 2020·Computational and Structural Biotechnology Journal·Xiaokang ZhangShihua Zhou

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