uORF-mediated translation allows engineered plant disease resistance without fitness costs

Nature
Guoyong XuXinnian Dong

Abstract

Controlling plant disease has been a struggle for humankind since the advent of agriculture. Studies of plant immune mechanisms have led to strategies of engineering resistant crops through ectopic transcription of plants' own defence genes, such as the master immune regulatory gene NPR1 (ref. 1). However, enhanced resistance obtained through such strategies is often associated with substantial penalties to fitness, making the resulting products undesirable for agricultural applications. To remedy this problem, we sought more stringent mechanisms of expressing defence proteins. On the basis of our latest finding that translation of key immune regulators, such as TBF1 (ref. 3), is rapidly and transiently induced upon pathogen challenge (see accompanying paper), we developed a 'TBF1-cassette' consisting of not only the immune-inducible promoter but also two pathogen-responsive upstream open reading frames (uORFsTBF1) of the TBF1 gene. Here we demonstrate that inclusion of uORFsTBF1-mediated translational control over the production of snc1-1 (an autoactivated immune receptor) in Arabidopsis thaliana and AtNPR1 in rice enables us to engineer broad-spectrum disease resistance without compromising plant fitness in the laboratory or ...Continue Reading

References

Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Aug 8, 2001·The Plant Journal : for Cell and Molecular Biology·M S ChernP C Ronald
Feb 18, 2004·Molecular Plant-microbe Interactions : MPMI·Heather A FitzgeraldPamela C Ronald
Jun 1, 2005·Trends in Biotechnology·Sarah J Gurr, Paul J Rushton
Jun 1, 2005·Trends in Biotechnology·Sarah J Gurr, Paul J Rushton
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Nov 6, 2007·Molecular Plant-microbe Interactions : MPMI·Yongqing LiJian Hua
Apr 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sarah E CalvoVamsi K Mootha
May 1, 2009·Plant Physiology·Fatemeh RahmaniJohannes Hanson
Jan 17, 2012·Current Biology : CB·Karolina M Pajerowska-MukhtarXinnian Dong
Feb 5, 2013·Annual Review of Plant Biology·Zheng Qing Fu, Xinnian Dong
Feb 23, 2013·Cold Spring Harbor Symposia on Quantitative Biology·K C M JohnsonX Li
Aug 21, 2013·Science·Jeffery L DanglBrian J Staskawicz
Apr 30, 2014·Molecular Plant·Bethany HuotSheng Yang He
Apr 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Manuel BenedettiGiulia De Lorenzo
Nov 18, 2015·Molecular Plant Pathology·Houxiang KangGuo-Liang Wang
Jul 28, 2016·Plant Science : an International Journal of Experimental Plant Biology·Kutubuddin Ali MollaKarabi Datta

❮ Previous
Next ❯

Citations

Aug 2, 2017·Proteomics·Polly Yingshan Hsu, Philip N Benfey
Aug 18, 2018·Science·R H Ramírez-GonzálezC Uauy
Jun 30, 2018·Molecular Plant-microbe Interactions : MPMI·Yuanzheng WangAlberto P Macho
Jan 24, 2019·Expert Review of Proteomics·Xinqiang YinHanmei Xu
Mar 23, 2019·Plant Signaling & Behavior·Shugang HuiMeng Yuan
Aug 2, 2017·Annual Review of Cell and Developmental Biology·Serge PlazaFrançois Payre
Nov 7, 2019·Nature·Julia Bailey-SerresJulian I Schroeder
Nov 27, 2019·International Journal of Molecular Sciences·Camila C FilgueirasDenis S Willett
Sep 11, 2019·Nucleic Acids Research·Mona Wu OrrShu-Bing Qian
Mar 14, 2020·Database : the Journal of Biological Databases and Curation·Ruixia NiuGuoyong Xu
Mar 21, 2020·Annual Review of Plant Biology·Wei LiGuo-Liang Wang
Sep 23, 2020·PloS One·Sharifa G CrandallDenis S Willett
Aug 13, 2020·Molecular Plant-microbe Interactions : MPMI·Solhee InDoil Choi
Oct 17, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yongping LiChunying Kang
May 26, 2018·The Plant Journal : for Cell and Molecular Biology·Andrew FunkJ Mitchell McGrath
Sep 8, 2018·Science·George H Greene, Xinnian Dong
Aug 21, 2019·Communications Biology·Xiaoyu LiuKarolina M Pajerowska-Mukhtar
Sep 5, 2020·Genome Biology·Sinian XingCaixia Gao
Sep 3, 2020·International Journal of Molecular Sciences·Ting ZhangYu Zhao
Feb 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yinggen KeShiping Wang
Jan 30, 2019·Frontiers in Plant Science·Viswanathan ChandranWen-Ming Wang
Dec 8, 2019·Plant Biotechnology Journal·Kutubuddin A MollaKarabi Datta
Sep 20, 2018·Viruses·Shachinthaka D Dissanayaka MudiyanselageYing Wang
Jan 18, 2019·International Journal of Molecular Sciences·Rongzhi ZhangShancen Zhao
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim
Dec 7, 2019·F1000Research·Anuj SharmaFrank F White
May 18, 2017·Nature·Julia Bailey-Serres, Wenbo Ma
Jan 10, 2018·Frontiers in Plant Science·Ekaterina V SheshukovaYuri L Dorokhov
Aug 21, 2020·The Journal of Biological Chemistry·Adam R BenthamMark J Banfield

❮ Previous
Next ❯

Datasets Mentioned

BETA
KC614689.1
AF234296.1

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

BLAST
Clustlw2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Plant Science : an International Journal of Experimental Plant Biology
Kutubuddin Ali MollaKarabi Datta
Current Opinion in Plant Biology
Rainer P BirkenbihlImre E Somssich
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Yakupjan HaximYule Liu
© 2022 Meta ULC. All rights reserved