PMID: 9180207May 16, 1997Paper

Up- and down-regulation of calpain inhibitor polypeptide, calpastatin, in postischemic hippocampus

Neuroscience Letters
T C SaidoM Yokota

Abstract

Based on our previous observation that transient forebrain ischemia induces calpain-catalyzed proteolysis in gerbil hippocampus in a region-specific manner, we examined the effect of ischemia on the quantity and localization of the endogenous calpain-specific inhibitor protein, calpastatin, in the tissue. Brief (5 min) forebrain ischemia followed by reperfusion induced an overall increase of calpastatin immunoreactivity in hippocampus, particularly in pyramidal cells, in 4 h as analyzed by Western blotting and immunohistochemistry. The amount of calpastatin, however, decreased to the preischemic level and lower in 24 h to 7 days due to proteolysis except in CA2 showing continuously elevated calpastatin immunoreactivity. Because calpastatin is not only a potent inhibitor but also a preferred substrate for calpain and because CA2 neurons are less vulnerable to ischemic stress than the adjacent CA1 neurons, these observations imply involvement of calpastatin in calpain regulation as a bait substrate and, possibly, in neuroprotection under ischemic conditions. Calpastatin may participate in the stress responses together with the previously known ischemia-induced stress proteins such as heat shock proteins.

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Citations

Mar 19, 1999·Journal of Neuroscience Research·K KitagawaM Hori
Apr 5, 2005·Brain Research·Atsushi KambeTakashi Watanabe
May 10, 2003·Brain Research. Brain Research Reviews·Swapan K RayNaren L Banik
Jan 9, 2007·Neurobiology of Disease·Georg Johannes MüllerFlemming Fryd Johansen
Aug 10, 2004·The Journal of Comparative Neurology·Sabine LadrechMarc Lenoir
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Pallavi B LimayeHarihara M Mehendale
Aug 30, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexandre BriguetThomas Meier
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Aug 15, 1998·Archives of Biochemistry and Biophysics·K K WangH Allen
Jul 2, 1998·Biochemical and Biophysical Research Communications·Y KourokuT Momoi

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