Abstract
Based on our previous observation that transient forebrain ischemia induces calpain-catalyzed proteolysis in gerbil hippocampus in a region-specific manner, we examined the effect of ischemia on the quantity and localization of the endogenous calpain-specific inhibitor protein, calpastatin, in the tissue. Brief (5 min) forebrain ischemia followed by reperfusion induced an overall increase of calpastatin immunoreactivity in hippocampus, particularly in pyramidal cells, in 4 h as analyzed by Western blotting and immunohistochemistry. The amount of calpastatin, however, decreased to the preischemic level and lower in 24 h to 7 days due to proteolysis except in CA2 showing continuously elevated calpastatin immunoreactivity. Because calpastatin is not only a potent inhibitor but also a preferred substrate for calpain and because CA2 neurons are less vulnerable to ischemic stress than the adjacent CA1 neurons, these observations imply involvement of calpastatin in calpain regulation as a bait substrate and, possibly, in neuroprotection under ischemic conditions. Calpastatin may participate in the stress responses together with the previously known ischemia-induced stress proteins such as heat shock proteins.
References
Sep 1, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J KawagoeK Kogure
Jan 1, 1992·Journal of Biochemistry·T C SaidoK Suzuki
Apr 29, 1991·Neuroscience Letters·K AbeK Kogure
Jul 17, 1989·Brain Research·P SeubertG Lynch
Nov 1, 1985·Journal of Neurochemistry·T S Nowak
May 6, 1982·Brain Research·T Kirino
Oct 20, 1995·The Journal of Biological Chemistry·A EtoS Kawashima
Oct 1, 1995·Stroke; a Journal of Cerebral Circulation·M YokotaK Suzuki
Oct 24, 1994·FEBS Letters·Y SaitoS Kawashima
Jun 1, 1994·Journal of Biochemistry·S NagaoS Kawashima
Apr 18, 1994·FEBS Letters·H SorimachiK Suzuki
Jul 2, 1993·Brain Research·S NishiK Nagata
Citations
Jun 22, 2000·The Journal of Pathology·R KakkarR K Sharma
Mar 19, 1999·Journal of Neuroscience Research·K KitagawaM Hori
Apr 5, 2005·Brain Research·Atsushi KambeTakashi Watanabe
Nov 25, 2003·Neuroscience Research·A RamiJ Winckler
May 29, 2000·Brain Research·A RamiJ Winckler
May 10, 2003·Brain Research. Brain Research Reviews·Swapan K RayNaren L Banik
Nov 7, 1998·Brain Research. Developmental Brain Research·K UraseT Momoi
Jun 7, 2000·Progress in Neurobiology·T Yamashima
Jun 28, 2003·Neurobiology of Disease·A Rami
Jul 4, 2009·Neurocirugía·A ColakO Celik
Nov 30, 2013·Amino Acids·Toru ImaiYoshihisa Ito
Aug 18, 2012·Neuroscience Letters·Wilasinee SuwanjangBanthit Chetsawang
Jan 9, 2007·Neurobiology of Disease·Georg Johannes MüllerFlemming Fryd Johansen
Jan 3, 2001·Cell Calcium·R KakkarR K Sharma
May 13, 1999·The Journal of Biological Chemistry·K BlomgrenH Hagberg
Nov 7, 2017·Journal of Experimental Neuroscience·Jirapa ChetsawangBanthit Chetsawang
Aug 10, 2004·The Journal of Comparative Neurology·Sabine LadrechMarc Lenoir
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Pallavi B LimayeHarihara M Mehendale
Feb 5, 2005·The Journal of Biological Chemistry·Jiro TakanoTakaomi C Saido
Aug 30, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexandre BriguetThomas Meier
Feb 10, 2006·The Journal of Pharmacology and Experimental Therapeutics·Feng HanKohji Fukunaga
Sep 1, 2000·American Journal of Physiology. Renal Physiology·Y ShiC L Edelstein
Jul 21, 2001·Annals of the New York Academy of Sciences·S K RayN L Banik
Nov 8, 2006·Neurochemical Research·L García-BonillaM Salinas
Aug 15, 1998·Archives of Biochemistry and Biophysics·K K WangH Allen
Jul 2, 1998·Biochemical and Biophysical Research Communications·Y KourokuT Momoi