Abstract
Chronic hepatitis B virus infection is associated with a high risk of developing into hepatocellular carcinoma, while tumor recognition is important during the immune surveillance process that prevents cancer development in humans. The mechanisms of immune evasion and the role of the early immune response in chronic infection caused by hepatitis B virus (HBV) are still unclear. In the present study, 1 copy or 1.2 copies of HBV genome was transfected into a hepatocellular carcinoma cell line BEL7405. RT-PCR, Western blot and flow cytometry analysis were used to evaluate the expression of HLA class I molecules and transporter associated with antigen processing 1 (TAP1). Finally, the cytotoxic activity of natural killer (NK) cells against HBV transfected liver cells was detected by MTT colorimetry method. Following transfection of 1 copy or 1.2 copies of HBV genome, HLA class I expression was up-regulated in BEL7405 cell line in a dose-dependent manner. Furthermore, increased the surface HLA class I expression were caused by enhanced expression of TAP1 at mRNA and protein levels in those transfected cells. Consequently, a significantly down-regulated cytotoxic activity of NK cells against HBV transfected liver cells was observed. ...Continue Reading
References
Jan 1, 1994·Critical Reviews in Immunology·P Le Bouteiller
Jan 14, 1998·International Journal of Cancer. Journal International Du Cancer·D P SingalD Bienzle
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·P PaulE D Carosella
May 13, 1999·Immunological Reviews·P Le Bouteiller, A Blaschitz
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Z X LiuG Dennert
Oct 20, 2000·Oncogene·N T GeorgopoulosG E Blair
Feb 13, 2001·Biochemical and Biophysical Research Communications·M MatsuiT Akatsuka
Mar 15, 2002·The Lancet Infectious Diseases·Maria-Christina Jung, Gerd R Pape
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Craig I FineJane A McCutcheon
May 20, 2003·Immunology and Cell Biology·Nicholas J C King, Alison M Kesson
Jul 15, 2003·Journal of Virology·Kerstin HerzerPeter H Krammer
Jul 26, 2003·Immunology Letters·Lorenzo MorettaAlessandro Moretta
Mar 31, 2005·Advances in Cancer Research·Chien-Chung ChangSoldano Ferrone
Feb 9, 2008·Tissue Antigens·A DiepstraA van den Berg
May 12, 2009·Molecular Immunology·Yuqing ShenWei Xie
Aug 12, 2009·Human Immunology·Fanny LeBouderBéatrice Riteau
Sep 15, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mingus J J RoseAnthony P Schwarer