Up-regulated microRNA-299 corrected with poor prognosis of glioblastoma multiforme patients by targeting ELL2

Japanese Journal of Clinical Oncology
Qian HuangChun Xie

Abstract

An increasing understanding of the genes and molecular pathways of glioblastoma multiforme (GBM) can provide us a useful insight for the development of more effective targeted therapeutic. To investigate the expression and clinical significance of miR-299 and its target genes in GBM, the expression levels of miR-299 and its target gene in human normal brain tissues and GBM were analyzed in silico using genes microarray and hierarchical clustering analysis followed by validation with quantitative RT-PCR. Our results show that miR-299 is up-regulated in GBM patients. Moreover, patients with low miR-299 expression had longer overall survival (OS) compared with those with high miR-299 expression. RNA polymerase II elongation factor, ELL2, was identified as a miR-299 direct target. High expression of ELL2 together with miR-299 down-regulation correlated with a shorter median OS. Our results provide the first evidence that ELL2 is a direct target of miR-299 and increased ELL2 expression and down-regulation of miR-299 are associated with GBM progression and poor prognosis in patients, suggesting that ELL2 and miR-299 might have potential prognostic value and be used as tumor biomarkers for the diagnosis of patients with GBM.

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Citations

Aug 8, 2019·Cancer Medicine·Xinqi JiaShan Yu
Oct 9, 2020·Asia-Pacific Journal of Clinical Oncology·Tayyebali SalmaniReza Akbarzadeh
Nov 19, 2017·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Yi-Hsuan WuJohn S Witte
Aug 22, 2021·NPJ Systems Biology and Applications·Joshua J LevyBrock C Christensen
Oct 4, 2020·Experimental and Molecular Pathology·Omidvar RezaeiSoudeh Ghafouri-Fard

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