Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina

Molecular and Cellular Neurosciences
Seifollah AzadiPer Ekström

Abstract

The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCdelta, mu and theta. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCdelta, mu and theta in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.

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Citations

Nov 5, 2008·Molecular Neurobiology·Javier Sancho-PelluzFrançois Paquet-Durand
Mar 3, 2011·Cell Death & Disease·J Sancho-PelluzP Ekström
Feb 9, 2013·Cell Death & Disease·A SahabogluF Paquet-Durand
Aug 19, 2007·Nucleic Acids Research·Stefano CalzaYudi Pawitan
Dec 6, 2014·Cell Death & Disease·P FarinelliP A R Ekström
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chun-tao ZhaoXiao-bing Yuan
Dec 13, 2006·Brain Research·Seifollah AzadiTheo van Veen
Jun 26, 2013·Journal of Neurochemistry·Pietro FarinelliPer A R Ekström
Nov 14, 2014·PloS One·Blanca Arango-GonzalezFrançois Paquet-Durand
Jul 13, 2016·Human Molecular Genetics·Manoj KulkarniFrançois Paquet-Durand
Dec 23, 2016·Scientific Reports·Ayse SahabogluFrançois Paquet-Durand
Dec 16, 2006·Journal of Neuroscience Research·François Paquet-DurandPer Ekström
Feb 4, 2009·Journal of Neurochemistry·François Paquet-DurandPer Ekström
Sep 3, 2010·Journal of Neurochemistry·François Paquet-DurandPer Ekström
Aug 3, 2019·Progress in Retinal and Eye Research·Michael PowerFrançois Paquet-Durand

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