PMID: 9172829Jan 1, 1997Paper

Up-regulation of action mRNA and reorganization of the cytoskeleton in Entamoeba histolytica trophozoites

The Journal of Eukaryotic Microbiology
R Manning-Cela, I Meza

Abstract

Actin mRNA levels were measured in Entamoeba histolytica trophozoites after experimentally inducing changes in the organization of the cytoskeleton. The treatment of trophozoites with forskolin, N6,2'-O-dibutyryl-adenosine 3':5'-cyclic monophosphate, and phorbol myristate acetate induced the organization of actin into multiple dots and defined structures with a concomitant increase in F-actin content. Cytochalasin D elicited polarization of the structured actin and formation of aggregates, as well as an increment in F-actin. Simultaneously, up-regulation of actin mRNA levels was produced by all the drugs. De novo synthesis of actin mRNA, as measured by nuclear run-ons, showed increased transcription of actin mRNA. On the other hand, treatment of cells with actinomycin D blocked the elevation of actin mRNA synthesis induced by forskolin, dibutyryl cyclic AMP, or cytochalasin D whereas, the increment induced by PMA was not affected. These data indicate a regulatory control of actin mRNA synthesis at the transcriptional level by forskolin, dibutyryl cyclic AMP and cytochalasin D, and transcriptional as well as post-transcriptional controls by phorbol myristate acetate. The experiments presented here suggest the possibility that, r...Continue Reading

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Citations

Apr 3, 2003·International Journal for Parasitology·Stepanka VanacovaPatricia J Johnson
Nov 9, 2000·Archives of Medical Research·A Valdéz-AguirreM Morales-Vallarta
May 16, 2002·Molecular Microbiology·Iris BruchhausEgbert Tannich
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