Up-regulation of acyl-coenzyme A:cholesterol acyltransferase-1 by transforming growth factor-beta1 during differentiation of human monocytes into macrophages

Biochemical and Biophysical Research Communications
Masaharu HoriS Horiuchi

Abstract

Expression of acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1) increases during differentiation of human monocytes into macrophages. To further elucidate the mechanism for ACAT-1 regulation in macrophages, we examined the effects of five cytokines including transforming growth factor-beta1 (TGF- beta1) on ACAT-1 expression in cultured human monocyte-macrophages. Immunoblot analyses showed that TGF-beta1 increased ACAT-1 protein expression by two- to threefold when added during differentiation of human monocytes into macrophages. ACAT activity increased in parallel by 1.8-fold. Northern blot analyses revealed that among the three ACAT-1 mRNA transcripts detected (2.8-, 3.6-, and 4.3-kb), the 2.8- and 3.6-kb transcripts were selectively increased by TGF-beta1. When TGF-beta1 was added after differentiation, ACAT-1 expression was not altered. Since TGF-beta1 is expressed in human atherosclerotic lesions, the current results suggest that ACAT-1 expression in monocytes infiltrating from the circulation to vascular walls may be enhanced by pre-existing TGF-beta1.

References

Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R K AssoianM B Sporn
May 1, 1997·Cardiovascular Research·X L WangD E Wilcken
Jan 1, 1997·Annual Review of Biochemistry·T Y ChangD Cheng
Oct 3, 1998·The Journal of Biological Chemistry·P OelkersS L Sturley
Oct 9, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·A MiyazakiT Y Chang
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·G Draude, R L Lorenz
May 4, 2000·The New England Journal of Medicine·G C BlobeH F Lodish
Jun 10, 2000·The Journal of Biological Chemistry·C C ChangT Y Chang
Oct 18, 2000·Biochemical and Biophysical Research Communications·N OhgamiH Nakayama
Feb 13, 2001·The Journal of Clinical Investigation·S FazioR V Farese
May 16, 2001·Current Opinion in Lipidology·T Y ChangA Miyazaki
Nov 2, 2002·Nature Medicine·Andrew C Li, Christopher K Glass
Jul 23, 2008·Perspectives in Vascular Surgery and Endovascular Therapy·Kimon Bekelis, Nicos Labropoulos

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Citations

Aug 31, 2006·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Takuya WatanabeTakashi Katagiri
Oct 31, 2008·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Tomoko KanomeAkira Miyazaki
Nov 5, 2019·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Han-Jen LinShih-Torng Ding

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