Up-regulation of apoptosis by gonadotrophin-releasing hormone agonist in cultures of endometrial cells from women with symptomatic myomas

Human Reproduction
Jia-Li KangJia Yu

Abstract

The aim of the present study was to evaluate the effect of gonadotrophin-releasing hormone agonist (GnRH-a), which is widely used in the medical treatment of symptomatic myomas, on the rate of endometrial cell apoptosis in cultures from women with symptomatic myomas. The study included 36 women with symptomatic myomas without endometrial hyperplasia or endometrial carcinoma, and 22 controls. Endometrial biopsy specimens were obtained from all subjects. Levels of apoptosis were examined in epithelial endometrial cell cultures before and after incubation with GnRH-a (triptorelin). The percentage of apoptotic cells was evaluated using the terminal deoxynucleotidyl transferase-mediated d-UTP nick end labeling assay and flow cytometry was used to evaluate Annexin V levels. Levels of spontaneous apoptosis were significantly lower in endometrial cultures from patients with symptomatic myomas than in those from control subjects (P < 0.01). Concentrations as low as 10(-7) M GnRH-a enhanced apoptosis in endometrial cultures from patients with symptomatic myomas (3.48% +/- 0.27% apoptotic cells in untreated samples and 25.45 +/- 0.95% in cells treated with 10(-7) M GnRH-a; P <0.01). The percentage of apoptotic cells also increased when cu...Continue Reading

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Citations

Nov 5, 2014·Journal of Translational Medicine·Huinan WengYifeng Wang
Mar 12, 2016·Hormone Molecular Biology and Clinical Investigation·Areege KamalDharani K Hapangama
Aug 19, 2015·Molecular and Cellular Endocrinology·Madelaine Cho-ClarkT John Wu
Oct 13, 2017·Biology of Reproduction·Lusine AghajanovaLinda C Giudice
Dec 22, 2016·Archives of Gynecology and Obstetrics·Lena Sagi-DainMartha Dirnfeld

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis