Up-regulation of CTD-2547G23.4 in hepatocellular carcinoma tissues and its prospective molecular regulatory mechanism: a novel qRT-PCR and bioinformatics analysis study

Cancer Cell International
Dong-Yue WenGang Chen

Abstract

Dysregulated expression of long non-coding RNAs (lncRNAs) has been reported in the pathogenesis and progression of multiple cancers, including hepatocellular carcinoma (HCC). LncRNA CTD-2547G23.4 is a novel lncRNA, and its role in HCC is still unknown. Here, we aimed to clarify the expression pattern and clinical value of CTD-2547G23.4 and to investigate the prospective regulatory mechanism via bioinformatics analysis in HCC. To identify differentially expressed lncRNAs in HCC, we downloaded RNA-Seq data for HCC and adjacent non-tumour tissues via The Cancer Genome Atlas (TCGA). CTD-2547G23.4 was selected by using the R language and receiver operating characteristic curve analysis. Furthermore, we validated the differential expression of CTD-2547G23.4 via Gene Expression Omnibus (GEO), ArrayExpress, Oncomine databases and quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between the CTD-2547G23.4 level and clinic pathological parameters was also assessed. To further probe the role of CTD-2547G23.4 in HCC cell cycle, lentivirus-mediated small interfering RNA was applied to silence CTD-2547G23.4 expression in Huh-7 cell line. In addition, the related genes of CTD-2547G23.4 gathered from The Atlas of Non...Continue Reading

References

Apr 24, 2002·Experimental Cell Research·Giuseppe ServilloPaolo Sassone-Corsi
May 22, 2002·Biochimica Et Biophysica Acta·Margaret C Frame
Feb 20, 2004·Cancer Research·Rinat AbramovitchAlik Honigman
Apr 3, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Scott M Dehm, Keith Bonham
Apr 26, 2011·European Journal of Cell Biology·Daisuke YamasakiTakefumi Doi
Dec 4, 2013·Nature Reviews. Genetics·Alessandro Fatica, Irene Bozzoni
Sep 28, 2014·Best Practice & Research. Clinical Gastroenterology·Cristina BosettiCarlo La Vecchia
Apr 10, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Lin-Jiang PanGang Chen
Sep 12, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jun LiBeicheng Sun
Jul 28, 2016·The Turkish Journal of Gastroenterology : the Official Journal of Turkish Society of Gastroenterology·Harun Erdalİbrahim Doğan
Sep 30, 2016·Journal of Hepatology·Alessandro VitaleTimothy M Pawlik
Dec 28, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Dan LiQimin Zhan
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 12, 2017·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·C ZhengJ Shao
Mar 12, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jinxia LiuRunzhou Ni
Mar 30, 2017·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·Hong-Mei LiJun-Qiang Chen

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE49713
CTD-2547G23.4
GSE27462

Methods Mentioned

BETA
RNA-Seq
chip
surgical resection
PCR7900
PCR

Software Mentioned

Ensemble
STRING
DAVID
TANRIC
GraphPad Prism
STATA
ggplot2
Cytoscape
R
SPSS

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.