Up-regulation of CYP2A5 expression by porphyrinogenic agents in mouse liver

Naunyn-Schmiedeberg's Archives of Pharmacology
P SalonpääH Raunio

Abstract

Coumarin 7-hydroxylase (COH) activity is catalyzed by the Cyp2a-5 gene product (CYP2A5 enzyme) in mice. Mouse hepatic CYP2A5 expression is often increased in conditions in which other P450 forms are repressed, e.g. after the administration of heavy metals and other toxic agents known to affect cellular heme balance. In this study, the effect of various porphyrinogenic chemicals on the expression CYP2A5 and the key enzymes in heme metabolism was studied. Administration of single doses of griseofulvin (1000 mg/kg), thioacetamide (10 mg/kg) and aminotriazole (1000 mg/kg) to DBA/2 and C57BL/6 mice produced up to 10-fold increases in hepatic COH catalytic activity. Dramatic, up to 130-fold increases in response to the inducers was observed in the amount of CYP2A5 steady-state mRNA. The mRNA contents of aminolevulinate synthase, ferrochelatase and heme oxygenase were also increased to a variable extent, possibly reflecting feed-back regulatory mechanisms. In D2 mice the CYP2A5 inducing effect of aminotriazole and thioacetamide, but not that of griseofulvin, pyrazole and phenobarbital, was abolished by exogenously administered heme arginate. In the B6 strain heme arginate treatment increased CYP2A5 expression but it did not affect the...Continue Reading

Citations

Sep 15, 2004·Toxicology and Applied Pharmacology·Ting Su, Xinxin Ding
Feb 21, 2002·Biochemical Pharmacology·Sami TetriHannu Raunio
Apr 1, 1997·General Pharmacology·A M BuzalehA M del Carmen Batlle
Mar 24, 2010·Malaria Journal·Ana Cecilia A X De-OliveiraFrancisco J R Paumgartten
Feb 13, 2001·Biochemical and Biophysical Research Communications·P ViitalaH Raunio
Jan 9, 1998·Drug Metabolism Reviews·P Honkakoski, M Negishi
Nov 1, 2009·Environmental Toxicology and Pharmacology·Roongnapa ApinanKesara Na-Bangchang
Sep 27, 2005·Biochemical Pharmacology·A'edah Abu-BakarMatti A Lang
Jul 7, 2007·Critical Reviews in Toxicology·Yasuhiro Masubuchi, Toshiharu Horie
Sep 3, 1998·Molecular Carcinogenesis·U M WastlR Schulte-Hermann
Sep 14, 2015·Naunyn-Schmiedeberg's Archives of Pharmacology·Feng HongYongke Lu

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