Up-regulation of cytochrome P450 and phase II enzyme systems in rat precision-cut rat lung slices by the intact glucosinolates, glucoraphanin and glucoerucin.

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
Ahmad F Abdull RazisCostas Ioannides

Abstract

It is believed that the chemopreventive activity of cruciferous vegetables in the lung and other tissues is exclusively the result of exposure to degradation products of glucosinolates, such as the isothiocyanates, and that the parent glucosinolates make no contribution. In the present study, evidence is presented for the first time that, in rat lung, the intact glucosinolates, glucoraphanin and glucoerucin, can modulate carcinogen-metabolising enzyme systems. The glucosinolates were isolated from cruciferous vegetables and incubated (1-25 μM) with precision-cut rat lung slices for 24h. Both glucosinolates, at concentrations as low as 1 μM, up-regulated the O-deethylation of ethoxyresorufin and the apoprotein levels of CYP1A1 and CYP1B1; supplementation of the incubation medium with myrosinase, the enzyme that converts glucosinolates to their corresponding isothiocyanates, abolished the rise in ethoxyresorufin O-deethylase activity. In contrast, neither glucosinolate, at the concentrations studied, influenced quinone reductase activity in the lung slices, but addition of myrosinase to the glucosinolate incubations led to a marked rise in activity. Glutathione S-transferase activity, monitored using 1-chloro-2,4-dinitrobenzene a...Continue Reading

References

Aug 1, 1990·Archives of Biochemistry and Biophysics·A PessinaP L Luisi
Dec 31, 1985·Biochemical and Biophysical Research Communications·Y C AwasthiH Mukhtar
May 20, 1999·Annual Review of Pharmacology and Toxicology·J P Whitlock
Mar 10, 2000·Cancer Causes & Control : CCC·L E VoorripsP A van den Brandt
Nov 25, 2000·Toxicology·C Lerche-Langrand, H J Toutain
Oct 23, 2002·Critical Reviews in Toxicology·Janne HukkanenHannu Raunio
May 27, 2004·Natural Product Reports·Birgit Holst, Gary Williamson
Dec 8, 2004·Current Topics in Medicinal Chemistry·Costas Ioannides, David F V Lewis
Feb 5, 2005·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M UmachandranC Ioannides
May 21, 2005·International Journal of Cancer. Journal International Du Cancer·Victoria YoxallCostas Ioannides
Mar 21, 2007·Journal of Agricultural and Food Chemistry·Radha M Bheemreddy, Elizabeth H Jeffery
Oct 1, 2008·Chemico-biological Interactions·Natalya HanlonCostas Ioannides
Oct 22, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N Konsue, C Ioannides
Jan 7, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Tram Kim LamAnthony J Alberg

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Citations

Feb 18, 2016·Fitoterapia·Thangavelu Soundara RajanEmanuela Mazzon
Nov 23, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria GaluppoEmanuela Mazzon
May 4, 2013·CNS Neuroscience & Therapeutics·Sabrina GiacoppoEmanuela Mazzon
May 26, 2015·Nutrition·Michael J Glade, Michael M Meguid
Sep 15, 2015·Journal of Agricultural and Food Chemistry·Julia BudnowskiMichael Blaut
May 18, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Ahmad Faizal Abdull Razis, Noramaliza Mohd Noor
May 11, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Mario Negrette-GuzmánJosé Pedraza-Chaverri
Jun 30, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Costas Ioannides
Oct 4, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Jean-Paul MorinChristelle Monteil
May 29, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Donatella CanistroMoreno Paolini
Apr 26, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rosaliana LibroEmanuela Mazzon
Jul 5, 2011·Journal of the Science of Food and Agriculture·Federica PasiniLuigi Filippo D'Antuono
Dec 31, 2017·Molecular Nutrition & Food Research·Ahmad Faizal Abdull RazisCostas Ioannides
Jul 14, 2021·Advanced Drug Delivery Reviews·Mohammed Ali SeloCarsten Ehrhardt

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