Up-regulation of endothelin receptor function and mRNA expression in airway smooth muscle cells following Sephadex-induced airway inflammation

Basic & Clinical Pharmacology & Toxicology
Bengt W GranströmL Edvinsson

Abstract

The hypothesis that up-regulation of bronchial constrictor endothelin receptors in airway smooth muscle cells may contribute to hyperreactivity during airway inflammation was tested in the present study by quantitative endothelin receptor mRNA analysis and functional responses in ring segments of rat trachea and bronchi. Real time reverse transcription polymerase chain reaction was used to quantify endothelin receptor expression in rat airway smooth muscle cells following Sephadex-induced inflammation. Compared with controls, Sephadex-induced airway inflammation caused a significant increase (3.9 times P<0.05) of endothelin receptor type B mRNA expression in bronchial smooth muscle cells, but not in tracheal smooth muscle cells. Functional myograph studies of bronchial and tracheal ring segments without epithelium (mechanically denuded) revealed an increase of the maximum contractile effects of endothelin-1 (a dual agonist for both endothelin type A and B receptors) and sarafotoxin 6c (a selective agonist for endothelin B receptors) in bronchial smooth muscle cells in Sephadex-induced inflammation, but not in tracheal smooth muscle cells. The enhanced maximal responses of bronchial smooth muscle cells to endothelin-1 and sarafo...Continue Reading

References

Nov 16, 1992·Biochemical and Biophysical Research Communications·S E AnderssonJ M Lundberg
Mar 25, 1991·Biochemical and Biophysical Research Communications·D L WilliamsB V Clineschmidt
Nov 1, 1995·American Journal of Respiratory and Critical Care Medicine·R G GoldieD W Hay
Jan 1, 1995·Respiration; International Review of Thoracic Diseases·S E AnderssonJ M Lundberg
Mar 30, 1994·Biochemical and Biophysical Research Communications·T FukurodaM Nishikibe
Mar 1, 1993·The American Review of Respiratory Disease·L A LaitinenT Haahtela
Jan 1, 1995·Journal of Cardiovascular Pharmacology·K Hadj-KaddourC Chevillard
Sep 1, 1996·American Journal of Respiratory and Critical Care Medicine·J R Michael, B A Markewitz
Jan 1, 1996·Respiration; International Review of Thoracic Diseases·S E AnderssonJ M Lundberg
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·G W ChalmersN C Thomson
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·F FinsnesO H Skjønsberg
Aug 17, 1999·European Journal of Pharmacology·E UddmanL Edvinsson
May 17, 2003·Stroke; a Journal of Cerebral Circulation·Marie HenrikssonLars Edvinsson
Jun 8, 2004·Acta Physiologica Scandinavica·B GranströmL Edvinsson

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Citations

Jul 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lana E KandalaftGeorge Coukos
Sep 17, 2008·Rheumatology·K W Frommer, U Müller-Ladner

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