Up-regulation of hypertonicity-activated myo-inositol transporter SMIT1 by the cell volume-sensitive protein kinase SGK1.

The Journal of Physiology
Fabian KlausChristoph Boehmer

Abstract

Mechanisms of regulatory cell volume increase following cell shrinkage include accumulation of organic osmolytes such as betaine, taurine, sorbitol, glycerophosphorylcholine (GPC) and myo-inositol. Myo-inositol is taken up by the sodium-myo-inositol-transporter SMIT1 (SLC5A3) expressed in a wide variety of cell types. Hypertonicity induces the transcription of the SMIT1 gene upon binding of the transcription factor tonicity enhancer binding protein (TonEBP) to tonicity responsive enhancers (TonE) in the SMIT1 promoter region. However, little is known about post-translational regulation of the carrier protein. In this study we show that SMIT1 is modulated by the serum- and glucocorticoid-inducible kinase SGK1, a protein genomically up-regulated by hypertonicity. As demonstrated by two-electrode voltage-clamp in the Xenopus oocyte expression system, SMIT1-mediated myo-inositol-induced currents are up-regulated by coexpression of wild type SGK1 and constitutively active (S422D)SGK1 but not by inactive (K127N)SGK1. The increase in SMIT1 activity is due to an elevated cell surface expression of the carrier while its kinetic properties remain unaffected. According to the decay of SMIT1 activity in the presence of brefeldin A, SGK1 st...Continue Reading

References

Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·K R Spring, A W Siebens
Aug 1, 1986·The American Journal of Physiology·G N Somero
Sep 24, 1982·Science·P H YanceyG N Somero
Feb 1, 1994·The Journal of Experimental Zoology·A D MacknightR D Purves
Aug 2, 1996·The Journal of Biological Chemistry·J D FerrarisA García-Pérez
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S WaldeggerF Lang
Apr 18, 1998·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·F LangH Völkl
May 6, 1998·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·S WaldeggerF Lang
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·H MiyakawaH M Kwon
Jun 9, 2000·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·C A WagnerS Bröer
Mar 22, 2002·Pflügers Archiv : European journal of physiology·S WärntgesF Lang
Apr 19, 2002·Journal of the American Society of Nephrology : JASN·Jianghui HouRoger W Brown
Mar 2, 2005·Biochemical and Biophysical Research Communications·Christoph BoehmerFlorian Lang
Mar 18, 2005·Physiology·James A McCormickDavid Pearce
Apr 20, 2007·Journal of Neurochemistry·Jeyaganesh RajamanickamChristoph Boehmer

❮ Previous
Next ❯

Citations

Dec 31, 2009·The Journal of Membrane Biology·Mentor SopjaniFlorian Lang
Jul 9, 2009·Current Opinion in Nephrology and Hypertension·Florian LangVolker Vallon
Aug 6, 2010·American Journal of Physiology. Cell Physiology·Irina M ZemtsovaFlorian Lang
Dec 12, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Sobuj MiaFlorian Lang
Mar 31, 2015·FEBS Letters·Sabine Schneider
Jun 10, 2010·The Journal of Physiology·Florian LangUndine E Lang
Mar 28, 2013·Journal of Biomedical Materials Research. Part a·Yuguan ZeFashui Hong
Sep 26, 2013·Molecular & Cellular Proteomics : MCP·Dietmar KültzRomina Sacchi
Jun 12, 2016·Applied and Environmental Microbiology·Christian ManskeHubert Hilbi
Jul 23, 2008·Proteomics·Ken S LauJames W Dennis
Mar 10, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anja EylensteinFlorian Lang
Jan 24, 2014·International Journal of Molecular Medicine·Momoko YoshidaHiromichi Yamanishi
Sep 22, 2009·Expert Opinion on Therapeutic Targets·Florian LangVolker Vallon
Jun 14, 2017·Biopharmaceutics & Drug Disposition·Haoxun Wang, Guofeng You
Sep 9, 2015·The Biochemical Journal·Eva S SchweikhardChristine Ziegler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.