Up-regulation of KISS1 as a novel target of Let-7i in melanoma serves as a potential suppressor of migration and proliferation in vitro.

Journal of Cellular and Molecular Medicine
Haider A AlkafajiSiamak Sandoghchian Shotorbani

Abstract

Melanoma is a kind of skin cancer that is begun by the alteration of melanocytes. miRNAs are small non-coding RNA molecules that regulate a variety of biological processes. KISS1, the metastasis-suppressor gene, encodes kisspeptins which inhibits migration and proliferation of cancers. This study was aimed to determine the role of Let-7i and KISS1 in melanoma cell migration and proliferation. At first, the expression of Let-7i and KISS1 was determined in patients with melanoma. In the in vitro part of the study, Let-7i mimics were transfected and the impact of its restoration on target gene expression, proliferation, migration and apoptosis of SK-MEL-3 melanoma cell line was assessed by real-time PCR and Western blotting, MTT assay, wound-healing assay and flow cytometry, respectively. Besides, KISS1 inhibitor siRNA alone and along with Let-7i was transfected to determine their probable correlation. The results revealed that either Let-7i or KISS1 were down-regulated in patients with melanoma. The results obtained from the in vitro part of the study revealed that restoration of Let-7i reduced the expression of metastasis- and proliferation-related target genes. Moreover, it was revealed that up-regulation of Let-7i attenuated m...Continue Reading

References

Jul 16, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Isabelle Sansal, William R Sellers
Jul 11, 2006·The New England Journal of Medicine·Arlo J Miller, Martin C Mihm
Aug 19, 2007·Cancer Research·Charles D JohnsonFrank J Slack
Oct 16, 2009·International Journal of Cancer. Journal International Du Cancer·Imke SatzgerRalf Gutzmer
Feb 25, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Miguel F SeguraEva Hernando
Jul 8, 2010·Journal of Neuro-oncology·Soon-Tae LeeJae-Kyu Roh
Jan 3, 2012·Melanoma Research·Vanessa F BonazziNicholas K Hayward
Jun 14, 2012·Carcinogenesis·Miguel F SeguraEva Hernando
Oct 17, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Donald M MillerKara Sedoris
Feb 5, 2013·Advances in Experimental Medicine and Biology·Manfred Kunz
Oct 22, 2014·International Journal of Biological Sciences·Bing-Jia ChenWen Zhang
Jan 15, 2015·Melanoma Research·Yixiong ZhouXianqun Fan
Feb 12, 2015·Oncotarget·Anastassia SerguienkoElse Munthe
Aug 1, 2015·Molecular Cancer Therapeutics·Jeyshka M Reyes-GonzálezPablo E Vivas-Mejía
Aug 28, 2016·Surgical Oncology·Nicholas LatchanaWilliam E Carson
Jan 25, 2017·Oncotarget·Luigi FattoreGennaro Ciliberto
Jul 25, 2018·Frontiers in Endocrinology·Chien-Yu ChenBangyan L Stiles
Aug 21, 2018·Frontiers in Endocrinology·Stephania GuzmanMoshmi M Bhattacharya
Dec 12, 2018·Journal of Cellular Biochemistry·Navid ShomaliBehzad Baradaran
Mar 15, 2019·Cancers·Anna Gajos-Michniewicz, Malgorzata Czyz
May 11, 2019·Journal of Gastrointestinal Cancer·Nazila AlizadehBita Sepehri
May 24, 2019·Journal of Experimental & Clinical Cancer Research : CR·Huan TangJun Guo
Aug 2, 2019·Cancers·Francesca LuongoRuggero De Maria
Aug 31, 2019·Clinical and Translational Medicine·Evgeny ChirshevJuli J Unternaehrer
Oct 4, 2019·Cell Biology International·Morteza AkbariBehzad Baradaran
Nov 7, 2019·Journal of Cellular Physiology·Navid ShomaliFaroogh Marofi
Jan 14, 2020·Journal of Gastrointestinal Cancer·Rozita TamjidifarFarshad Mahdavi
Mar 11, 2020·Cancer Metastasis Reviews·Thuc LyDanny R Welch
May 12, 2020·Current Molecular Medicine·Navid ShomaliSiamak Sandoghchian Shotorbani
Jan 12, 2021·International Immunopharmacology·Mohammad Reza Mohammad Hoseini AzarSiamak Sandoghchian Shotorbani

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