Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

Bioscience Reports
Bao-Long PanJun-Long Liao

Abstract

Osteosarcoma (OS) is the most common histological form of primary bone cancer. It is most prevalent in teenagers and young adults. The present study aims at exploring the regulatory effect of microRNA-340 (miR-340) on OS cell proliferation, invasion, migration, and apoptosis via regulating the Notch signaling pathway by targeting β-catenin (cadherin-associated protein) 1 (CTNNB1). OS tissues belonging to 45 patients and normal femoral head tissues of 45 amputees were selected. Cells were allocated to different groups. In situ hybridization was performed to determine the positive rate of miR-340 expression while immunohistochemistry was used to determine that of CTNNB1 and B-cell lymphoma 2 (Bcl-2). We used a series of experiments to measure the expressions of related factors and assess rates of cell proliferation, migration, invasion, cycle, and apoptosis respectively. Our results show that miR-340 was expressed a higher level in normal tissue than OS tissue. Expression of Notch, CTNNB1, hairy and enhancer of split 1 (Hes1), Bcl-2, Runt-related transcription factor 2 (Runx2), and osteocalcin increased and that of miR-340, Bcl-2 interacting mediator of cell death (BIM), and Bcl-2 associated protein X (Bax) decreased in OS tissue...Continue Reading

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Citations

May 10, 2019·Current Osteoporosis Reports·Daniel W Youngstrom, Kurt D Hankenson
May 4, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Wenhui Zeng
Mar 25, 2021·Cancer Management and Research·Weizhu ZhaoLigang Xing
Jun 28, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chang SongXiao Zhu

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Methods Mentioned

BETA
X-ray
PCR
electrophoresis
transfection
flow cytometry
xenograft

Software Mentioned

SPSS

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