Up-regulation of miR-210 by vascular endothelial growth factor in ex vivo expanded CD34+ cells enhances cell-mediated angiogenesis.

Journal of Cellular and Molecular Medicine
Mohamad Amer AlaitiMarco A Costa

Abstract

Ex vivo culture has been proposed as a means to augment and repair autologous cells in patients with chronic diseases, but the mechanisms governing improvement in cell function are not well understood. Although microRNAs (miRs) are increasingly appreciated as key regulators of cellular function, a role for these factors in CD34+ cell-mediated angiogenesis has not been elucidated. Vascular endothelial growth factor (VEGF) was previously shown to induce expression of certain miRs associated with angiogenesis in endothelial cells and promote survival and number of vascular colony forming units of haematopoietic stem cells (HSCs). We sought to evaluate the role of VEGF in expansion and angiogenic function of CD34+ cells and to identify specific miRs associated with angiogenic properties of expanded cells. Umbilical cord blood CD34+ cells were effectively expanded (18- to 22-fold) in culture medium containing stem cell factor (SCF), Flt-3 ligand (Flt-3), thrombopoietin (TPO) and interleukin-6 (IL-6) with (postEX/+VEGF) and without VEGF (postEX/noVEGF). Tube formation in matrigel assay and tissue perfusion/capillary density in mice ischaemic hindlimb were significantly improved by postEX/+VEGF cells compared with fresh CD34+ and post...Continue Reading

References

Aug 23, 2000·The Journal of Clinical Investigation·G C SchattemanB A Christy
Dec 16, 2005·Nature·Leigh CoultasJanet Rossant
Aug 1, 2006·Nature Genetics·Michael DewsAndrei Thomas-Tikhonenko
Aug 31, 2006·Molecular and Cellular Biology·Anwar HossainGrady F Saunders
Feb 20, 2007·Journal of the American College of Cardiology·Eduard ShantsilaGregory Y H Lip
Mar 24, 2007·Circulation Research·Yajaira SuárezWilliam C Sessa
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Apr 18, 2008·The Journal of Biological Chemistry·Pasquale FasanaroFabio Martelli
Jun 24, 2008·Nature Cell Biology·Catherine LabbayeCesare Peschle
Aug 13, 2008·Developmental Cell·Jason E FishDeepak Srivastava
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yajaira SuárezWilliam C Sessa
Jan 6, 2009·Journal of Cellular and Molecular Medicine·Coen van SolingenAnton Jan van Zonneveld
Feb 21, 2009·Journal of Visualized Experiments : JoVE·Hiroshi NiiyamaJohn P Cooke
Jul 28, 2009·Nature Cell Biology·Sze Wan ShanBurton B Yang
Oct 15, 2009·The Journal of Biological Chemistry·Pasquale FasanaroFabio Martelli
Sep 21, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eunju OYoung Ae Joe
May 14, 2011·Circulation Research·Haruchika MasudaTakayuki Asahara
Jul 9, 2011·Circulation Research·Douglas W LosordoUNKNOWN ACT34-CMI Investigators
Aug 13, 2011·Circulation Research·Susmita SahooDouglas W Losordo

❮ Previous
Next ❯

Citations

Jan 8, 2014·Acta Biochimica Et Biophysica Sinica·Xin Huang, Jianhong Zuo
Sep 24, 2013·Antioxidants & Redox Signaling·Simona GrecoFabio Martelli
Aug 8, 2014·Experimental and Molecular Pathology·Hamidreza MaroofAlfred King-Yin Lam
Oct 12, 2013·Antioxidants & Redox Signaling·Harriet E GeeMircea Ivan
Oct 16, 2012·Seminars in Cell & Developmental Biology·Chandan K Sen, Sashwati Roy
Jun 10, 2014·Journal of Experimental & Clinical Cancer Research : CR·Qin QinLi Baosheng
Dec 18, 2013·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·R KarakoyunZ Sener
Oct 12, 2013·Journal of Cellular Biochemistry·Runting YinWei Zhang
Jan 3, 2014·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Nian-Sheng LaiYu-Chang Lin
Feb 11, 2015·Experimental and Therapeutic Medicine·Yan ZhuShengnian Zhou
Jul 1, 2016·Expert Opinion on Therapeutic Targets·Chun-Xia RenDong-Qing Ye
Aug 21, 2012·Human Reproduction·Luis A RamónJuan Gilabert-Estellés
Apr 5, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Heyam HayderChun Peng
Dec 12, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brian McNeillErik J Suuronen
Nov 1, 2015·Stem Cell Research & Therapy·Andrea Alex SchiavoMichela Pozzobon
Dec 28, 2019·International Journal of Molecular Sciences·Germana ZaccagniniFabio Martelli
Nov 14, 2012·The Journal of Biological Chemistry·Lucia CicchillittiFabio Martelli
Aug 27, 2017·Scientific Reports·G ZaccagniniF Martelli
Apr 30, 2019·BioMed Research International·Yanming ZhangLijun Hou
May 24, 2017·European Heart Journal Supplements : Journal of the European Society of Cardiology·Simona GrecoFabio Martelli
Oct 15, 2018·Cellular and Molecular Life Sciences : CMLS·Wanli YangDaiming Fan
Mar 1, 2019·Non-coding RNA·Jakub GodlewskiElzbieta Salinska
Apr 27, 2018·Frontiers in Physiology·Sveva BolliniOrnella Parolini
Sep 16, 2021·Antioxidants & Redox Signaling·Germana ZaccagniniFabio Martelli

❮ Previous
Next ❯

Methods Mentioned

BETA
density-gradient centrifugation
flow cytometry
fluorescence-activated cell sorting
PCR
transfection
light microscopy
FACS

Software Mentioned

Cell [UNK]
Photoshop

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.