Up-regulation of miR-325-3p suppresses pineal aralkylamine N-acetyltransferase (Aanat) after neonatal hypoxia-ischemia brain injury in rats

Brain Research
Yuanyuan YangXin Ding

Abstract

Survivors of hypoxic-ischemic brain damage (HIBD), besides impairment of psychomotor development, often develop circadian rhythm disorders, although the underlying mechanisms are largely unknown. Here, we first verified that mRNA and protein expression of pineal aralkylamine N-acetyltransferase (Aanat), a key regulator for melatonin (MT) synthesis, along with MT, were severely impaired after HIBD. In addition, we demonstrated that neonatal HIBD disrupted the circadian rhythmicity of locomotor activities in juvenile rats. Based on bioinformatics analysis of a high throughput screening of miRNA expression changes after HIBD (Ding et al., 2015), we identified one microRNA, miR-325-3p, as a potential candidate responsible for the down regulation of Aanat after HIBD. Luciferase reporter assays demonstrated a specific interaction between miR-325-3p and Aanat mRNA 3'-UTR. miR-325-3p blocked norepinephrine (NE) induced Aanat activation in cultured pinealocytes. In addition, miR-325-3p inhibition partially rescued Aanat induction by NE, which was significantly reduced under oxygen glucose deprivation. By elucidating the role of pineal miR-325-3p on Aanat expression upon injury, our study provides new insights into the pathophysiological...Continue Reading

Citations

Aug 10, 2018·Journal of Pineal Research·Lucas Sagrillo-FagundesCathy Vaillancourt
Nov 5, 2019·Journal of Cellular Biochemistry·Yuzhuo WangMin Hu
Feb 14, 2020·Artificial Cells, Nanomedicine, and Biotechnology·Yaqin CaoYubin Dong
Jun 11, 2020·Archives of Physiology and Biochemistry·Saptadip Samanta
Mar 28, 2019·International Journal of Endocrinology·Justyna PaprockaMaria Sokół
Jun 26, 2020·Frontiers in Neurology·Martín BusteloAntonio W Danilo Gavilanes
Mar 7, 2021·International Journal of Molecular Sciences·Dmitry O IvanovRuslan A Nasyrov
Jul 20, 2021·Experimental Neurology·Lixiao XuBin Sun

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