Up-regulation of phosphorylation of focal adhesion kinase and paxillin by combination of substance P and IGF-1 in SV-40 transformed human corneal epithelial cells

Biochemical and Biophysical Research Communications
M NakamuraTeruo Nishida

Abstract

We previously reported that substance P (SP) and insulin-like growth factor-1 (IGF-1) synergistically facilitate corneal epithelial migration in vitro and in vivo. We wanted to determine whether proteins responsible for cellular attachment are activated in corneal epithelial cells. To do this, we examined changes in tyrosine phosphorylation in focal adhesion kinase (FAK) and paxillin in cultured SV-40 transformed human corneal epithelial cells (HCE cells). HCE cells were cultured in the absence or presence of either SP (2 x 10(-5) M) or IGF-1 (10 ng/ml) or both SP and IGF-1. Treatment of HCE cells by either SP or IGF-1 alone did not alter tyrosine phosphorylation in either FAK or paxillin. However, the combination of SP and IGF-1 significantly increased tyrosine phosphorylation in both FAK and paxillin. In contrast, the combination of SP and IGF-1 was not observed to produce synergistic effects on the activation of mitogen-activated protein kinase in HCE. These results show that the synergistic effects of SP and IGF-1 on corneal epithelial wound healing were expressed through activation of the integrin, FAK, and paxillin system.

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Citations

Apr 5, 2001·American Journal of Physiology. Cell Physiology·S IkeoK Hashizume
Aug 3, 2012·Japanese Journal of Ophthalmology·Teruo Nishida
Nov 7, 2012·Journal of Cellular Physiology·Cheryl Chi, Vickery Trinkaus-Randall
Jan 11, 2020·Ophthalmic Plastic and Reconstructive Surgery·Ji Kwan ParkAndrea L Kossler
Aug 21, 2010·Eye & Contact Lens·Teruo Nishida
Mar 19, 2013·Journal of Biomedical Materials Research. Part a·Ikiru AtsutaKiyoshi Koyano
Jun 23, 2009·Current Opinion in Ophthalmology·Teruo Nishida, Ryoji Yanai
Jun 18, 2016·Cellular and Molecular Life Sciences : CMLS·Alireza MashaghiReza Dana
Feb 15, 2019·The British Journal of Ophthalmology·Shawn Rong LinJames Chodosh
Apr 18, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kimberly J PerryJonathan J Henry
Sep 8, 2018·Investigative Ophthalmology & Visual Science·Joseph CatapanoGregory H Borschel
Oct 12, 2013·Archives of Oral Biology·Ikiru AtsutaKiyoshi Koyano
Apr 17, 2003·Experimental Eye Research·Linda J MüllerTimo M T Tervo
Sep 8, 2009·Brain Research Bulletin·Fu-Shin X YuJenny Huang

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