Up-regulation of photoprotection and PSII-repair gene expression by irradiance in the unicellular green alga Dunaliella salina

Marine Biotechnology
Seunghye ParkEonSeon Jin

Abstract

The unicellular green alga Dunaliella salina is an attractive model organism for studying photoacclimation responses and the photosystem II (PSII) damage and repair process in the photosynthetic apparatus. Irradiance during cell growth defines both the photoacclimation and the PSII repair status of the cells. To identify genes specific to these processes, a cDNA library was created from irradiance-stressed D. salina. From the cDNA library, 1112 randomly selected expressed sequence tags (ESTs) were analyzed. Because ESTs constitute the expressed part of the genome, the strategy of randomly sequencing cDNA clones at their 5'-ends allowed us to obtain information about the transcript level of numerous genes in light-stressed D. salina. The results of a BLASTX search performed on the obtained total set of ESTs showed that approximately 1% of the ESTs could be assigned to genes coding for proteins that are known to be up-regulated in response to high-light stress. Specifically, after 48 h of high-light exposure of the cells, an increase in the expression level of antioxidant genes, such as Fe-SOD and APX, was observed, as well as elevated levels of the Cbr transcript, a light-harvesting Chl-protein homolog. Further, the ATP-dependen...Continue Reading

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Citations

Jan 5, 2014·The Journal of Microbiology·Hancheol JeonEonSeon Jin
Sep 13, 2011·Bioprocess and Biosystems Engineering·Wonduck KimSi Wouk Kim
Oct 23, 2015·Environmental Science and Pollution Research International·Lei ChenYiliang He
Aug 30, 2008·Trends in Biotechnology·Packo P LamersRené H Wijffels
Feb 28, 2009·Journal of Applied Microbiology·A Hosseini Tafreshi, M Shariati
May 29, 2013·Journal of Basic Microbiology·Shu ZhangDairong Qiao
Jun 6, 2016·Plant Physiology and Biochemistry : PPB·Xinyu ZhaoYing Wang
Dec 1, 2013·Botanical Studies·Tzan-Chain Lee, Ban-Dar Hsu
Apr 24, 2021·Life Sciences in Space Research·Bo WangGaohong Wang
Mar 31, 2015·Environmental Science & Technology·Sung-Gil HongJungbae Kim

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