Up-regulation of the pentose phosphate pathway and HIF-1α expression during neural progenitor cell induction following glutamate treatment in rat ex vivo retina

Cell Biology International
Kazuhiro TokudaKazuhiro Kimura

Abstract

The metabolic state influences the regulation of neural stem/progenitor cells. The pentose phosphate pathway (PPP), an alternative metabolic pathway that operates parallel to glycolysis, not only provides key intermediates for biosynthetic reactions but also controls the fate of neural stem/progenitor cells. We have previously shown that glutamate application leads to the induction of neural progenitor cells in mature ex vivo rat retina. In this study, we investigated whether regulation of the PPP might be changed following glutamate treatment of the retina. Immunoblot analysis revealed that the amount of glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the PPP as well as that of 6-phosphogluconate dehydrogenase (6PGD), another enzyme in this pathway, increased in the glutamate-treated retina. Consistent with the fact that both these enzymes generate reduced nicotinamide adenine dinucleotide phosphate (NADPH), the amount of NAPDH in the treated retina was significantly higher compared with that in the untreated retina. We also found that both DNA synthesis as well as the expression of fatty acid synthase (FASN) increased significantly in the glutamate-treated retina. Furthermore, hypoxia-inducible factor 1-...Continue Reading

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Feb 8, 2018·Cell Metabolism·Xinshou OuyangWajahat Zafar Mehal

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Citations

Jan 11, 2020·Frontiers in Oncology·Abraham Pedroza-TorresLuis A Herrera
Mar 4, 2021·International Journal of Molecular Sciences·Elisa MurenuStylianos Michalakis
Oct 25, 2020·Biomedical Journal·Hung-Chi YangDaniel Tsun-Yee Chiu

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