Up-regulation of tyrosine hydroxylase gene transcription by tetradecanoylphorbol acetate is mediated by the transcription factors Ets-like protein-1 (Elk-1) and Egr-1

Journal of Neurochemistry
Luisa StefanoGerald Thiel

Abstract

Tyrosine hydroxylase is the rate-limiting enzyme in the biosynthesis of catecholamines. Expression of the tyrosine hydroxylase gene is regulated at the transcriptional level by extracellular signalling molecules, including epidermal growth factor (EGF), nerve growth factor (NGF) and glucocorticoids. We have analysed the stimulation of tyrosine hydroxylase gene transcription by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in noradrenergic locus coeruleus-like CATH.a cells and observed a striking enhancement of the transcriptional activation potential of the ternary complex factor Ets-like protein-1 (Elk-1), a key transcriptional regulator of serum response element-driven gene transcription. Likewise, TPA strongly up-regulated the biosynthesis of the transcription factor Egr-1 via distal serum response elements within the Egr-1 5'-flanking region. Subsequently, enhancement of the transcriptional activation potential of Egr-1 was observed. Overexpression of Egr-1 was sufficient to activate transcription of a tyrosine hydroxylase promoter/reporter gene, corroborating the view that the tyrosine hydroxylase gene is a target gene of Egr-1. Expression of dominant-negative mutants of Elk-1 or Egr-1 impaired TPA-induced s...Continue Reading

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Citations

Jun 18, 2010·American Journal of Physiology. Cell Physiology·Amit K MitraIrving H Zucker
May 29, 2014·Journal of Neural Transmission·Izel TekinKent E Vrana
Mar 27, 2010·Cellular and Molecular Life Sciences : CMLS·Rui MengMathias Montenarh
Jun 5, 2012·Cell Calcium·Isabelle MüllerGerald Thiel
Aug 2, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Robert Lenartowski, Anna Goc
Jul 22, 2008·Biochemical and Biophysical Research Communications·Güralp O CeyhanKarl-Herbert Schäfer
Sep 25, 2008·Journal of Cellular Biochemistry·Sabine I MayerGerald Thiel
May 27, 2010·Journal of Cellular Biochemistry·Isabelle MüllerGerald Thiel
Nov 26, 2013·Neurobiology of Disease·Radosveta KoldamovaIliya Lefterov
Jan 24, 2017·Biochemical Pharmacology·Gerald Thiel, Oliver G Rössler
Jan 21, 2017·Journal of Cellular Physiology·Estelle R Simo-CheyouAshok K Srivastava
Sep 15, 2011·Endocrinology·Gerald Thiel, Oliver G Rössler
Jun 10, 2016·Journal of Cellular Biochemistry·Oliver G Rössler, Gerald Thiel
Aug 27, 2009·Journal of Cell Science·Sabine I MayerGerald Thiel
Jul 25, 2021·Biochemical Pharmacology·Gerald ThielOliver G Rössler

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