UPARSE: highly accurate OTU sequences from microbial amplicon reads

Nature Methods
Robert C Edgar

Abstract

Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with ≤1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community.

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Related Concepts

Gene Amplification Technique
Phylogeny
Metagenomics
Computer Programs and Programming
Sequencing
Microbial
Testicular Neoplasms
Gene Amplification Abnormality
RNA, Ribosomal, 16S
Online Mendelian Inheritance In Man

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