Update on the detection of beta-exotoxin in Bacillus thuringiensis strains by HPLC analysis

Journal of Applied Microbiology
Carmen Sara HernándezI Larget-Thiéry

Abstract

The current work aimed to study the presence of beta-exotoxin by high-performance liquid chromatography (HPLC) in supernatant fluids from final whole cultures of the 69 type strains and 13 subtypes of Bacillus thuringiensis strains, as well as from some insecticidal strains. Results from HPLC and bioassays with Ephestia kuhniella (Lepidoptera Pyralidae) were compared. Type I beta-exotoxin was only detected in type strains representing serotypes H1, H9 and H10a,10b. Discrepancies between HPLC and bioassays were found in H8a,8b and some insecticidal strains, which suggests the occurrence of another soluble toxin different from type I beta-exotoxin, possibly type II beta-exotoxin. This study shows the need to use bioassays to determine the presence of beta-exotoxin activity. However, HPLC is a fast and sensitive technique if only type I beta-exotoxin is to be determined. The occurrence of beta-exotoxin in a type strain does not imply production of this metabolite by other strains belonging to the same serovar. These results complete the characterization of type strains belonging to the International Entomopathogenic Bacillus Collection (Institut Pasteur, Paris, France).

Citations

Feb 13, 2003·Journal of Invertebrate Pathology·Carmen Sara HernándezJuan Ferré
Aug 30, 2002·Applied and Environmental Microbiology·Sylvain EspinasseVincent Sanchis
May 18, 2004·Journal of Invertebrate Pathology·Patricia Tamez-GuerraCristina Rodríguez-Padilla
Aug 19, 2014·Journal of Invertebrate Pathology·Diego H SaukaGraciela B Benintende

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