UPLC-HRMS-Based Plasma Metabolomic Profiling of Novel Biomarkers by Treatment with KDZI in Cerebral Ischemia Reperfusion Rats

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Chunguo WangBaosheng Zhao

Abstract

Kudiezi injection (KDZI), also known as Diemailing injection, is a traditional Chinese medicine injection of the composite plant Ixeris sonchifolia Hance (also known as Kudiezi), and has been widely used to treat coronary heart disease, angina pectoris, and cerebral infarction, but its pharmacological mechanisms remain unclear. This study is designed to explore the effects of KDZI on middle cerebral artery occlusion and reperfusion (MCAO/R) rats, and to identify metabolic features of cerebral ischemia reperfusion by using a nontargeted metabolic profiling method based on ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). In this process, 32 potential biomarkers were found in plasma. KDZI significantly upregulated the levels of taurochenodesoxycholic acid, leucine, l-phenylalanine, l-tryptophan, arachidonic acid (ARA), and phosphatidyl ethanolamines (PE), phosphatidyl cholines (PC) and downregulated the levels of l-valine and 5-hydroxyindole-3-acetic acid (5-HIAA) in plasma. The results indicated that the mechanisms of KDZI on MCAO/R were related to the mechanisms of amino acid and lipid metabolism.

References

Mar 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·B DjuricicL R Drewes
Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
Jan 1, 1981·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A TamuraG M Teasdale
Jul 18, 2002·Nature Reviews. Drug Discovery·Jeremy K NicholsonElaine Holmes
Apr 6, 2004·Brain Research. Brain Research Protocols·Chintamani Narasinh JoshiPuvvada Sri Ramachandra Murthy
Jun 23, 2004·International Journal of Clinical Practice·A DoganG Akhan
Jun 15, 2005·Molecular Neurobiology·Atsushi SaitoPak H Chan
May 23, 2006·Pharmaceutical Research·John C LindonJeremy K Nicholson
Apr 17, 2007·Lancet Neurology·Ming LiuLing-Zhi Kong
Feb 16, 2008·Molecular Systems Biology·Ville-Petteri MäkinenMika Ala-Korpela
Jul 7, 2011·Circulation·Anthony S Kim, S Claiborne Johnston
Dec 12, 2012·Nature Protocols·Elizabeth J WantJeremy K Nicholson
May 9, 2014·Journal of Chromatography. a·Shama NazCoral Barbas
Sep 6, 2014·Trends in Cell Biology·Atsuko Kasahara, Luca Scorrano
Apr 14, 2015·Expert Review of Neurotherapeutics·Tomasz Dziedzic
Nov 20, 2015·Neuropharmacology·Felicita PedataAnna Maria Pugliese
Jan 23, 2016·Journal of Hepatology·Mark J W McPhailSimon D Taylor-Robinson
May 11, 2016·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Fu-Qin ChenJing-Yan Han
Jan 7, 2017·BMC Complementary and Alternative Medicine·Xuemei LiuYunling Zhang
Apr 8, 2017·Saudi Journal of Biological Sciences·Xiaoli YanMingsan Miao
Nov 16, 2017·Nucleic Acids Research·David S WishartAugustin Scalbert

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA
deamination

Software Mentioned

SPSS
Sieve
Lipidbank
Pro Plus
MeV Multi Experiment Viewer
Image
Compound Discoverer
Mass Bank
mzCloud
SIMCA

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.