UPLC-MS/MS Determination of Twelve Ginsenosides in Shenfu Tang and Dushen Tang

International Journal of Analytical Chemistry
Dawai YangNa Guo

Abstract

Shenfu Tang and Dushen Tang (one of the composite medicines for Shenfu Tang) are widely used Traditional Chinese herbal formulations and ginsenosides are their main bioactive components. However, there are rare studies about simultaneous analysis of ginsenosides in Shenfu Tang and Dushen Tang. In order to identify ginsenosides in Shenfu Tang and Dushen Tang and to explore law of compatibility of medicines in the decoction, a method for simultaneous determination of twelve ginsenosides in Shenfu Tang and Dushen Tang was developed by ultraresolution liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). The method showed satisfactory linearity (r > 0.9915), repeatability (RSD < 9.58%), intra- and interday precisions (RSD<11.90%), and high yields of recovery (92.26-113.20%) for twelve major constituents, namely, ginsenosides-Rb1, Rb2, Rb3, Rc, Rd, Rg1, Re, Rf, Rg2, Rg3, Rh1, and F2. Furthermore, the concentration of twelve ginsenosides in Dushen Tang and Shenfu Tang was also simultaneously analyzed. Most of ginsenosides except Rg1 and Rb1 showed higher contents in Shenfu Tang compared to Dushen Tang. The compatibility of the formula had the effect of promoting or inhibiting the dissolution of some major componen...Continue Reading

References

Nov 26, 1999·Biochemical Pharmacology·A S AtteleC S Yuan
Nov 12, 2005·Analytical and Bioanalytical Chemistry·T LigorB Buszewski
Sep 6, 2008·Advances in Food and Nutrition Research·Lars P Christensen
Oct 22, 2008·Lancet·Jin-Ling TangKan-Wen Ma
Jul 3, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dawei YangNa Guo
Feb 21, 2018·Phytochemical Analysis : PCA·Wei WuShuying Liu

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