UPLC-Q-TOF-MS(E) analysis of the constituents of Ding-Zhi-Xiao-Wan, a traditional Chinese antidepressant, in normal and depressive rats

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lu XuPing Liu

Abstract

Ding-Zhi-Xiao-Wan (DZXW) is a traditional Chinese medicine widely used for treating depression. To clarify the bioactive constituents of DZXW, a new rapid ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS(E)) method was established in this study, with the whole extract of the formula separated into multiple components to facilitate the analytical process. In total, 97 compounds were detected and 88 were identified in DZXW. Based on their exact masses, fragmentation characteristics, and retention times, 85 of the 88 compounds were confirmed either conclusively or tentatively, and three potentially novel compounds were identified. In addition, following a three-day oral administration of DZXW, 60 and 28 compounds were observed in the plasma of normal and depressive rats, respectively. Finally, by combining our data with pharmacological information, 10 compounds were predicted as the likely bioactive constituents of DZXW as an antidepressant agent. Our approach provided a rapid method for characterising the chemical constituents of DZXW, and we were the first to screen for bioactive indexes in the plasma of depressive rats. Furthermore, our results provide useful chemical informatio...Continue Reading

References

Jul 9, 2010·European Journal of Pharmacology·Yu-Jing ChenLin Li
May 19, 2011·The Journal of Pharmacy and Pharmacology·Yuan HuKhalid Rahman
Jan 24, 2012·Journal of Pharmaceutical and Biomedical Analysis·Li-ping KangBai-ping Ma
Mar 1, 2012·Magnetic Resonance in Chemistry : MRC·Xiao-Jie LiuXue-Mei Qin
Jul 17, 2012·Fitoterapia·Chuangjun LiNaihong Chen
Feb 13, 2013·Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·Juan-Juan YanPing Liu
Mar 21, 2013·Journal of Pharmaceutical and Biomedical Analysis·Yanyan WangQinglin Zhang

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