UPLC-TOF-MS analysis of Galium spurium towards its neuroprotective and anticonvulsant activities

Journal of Ethnopharmacology
Nilüfer OrhanIlkay Erdogan Orhan

Abstract

Galium species have been reported to be used against epilepsy in traditional Turkish folk medicine. The present work was undertaken to evaluate the in vivo anticonvulsant and in vitro neuroprotective effects of Galium spurium L. and to determine the major constituents by UPLC-TOF-MS. Anticonvulsant activity of the aerial parts of Galium spurium was investigated using pentylenetetrazole, picrotoxin, and maximal electroshock-induced seizure animal models. In order to evaluate the safety, neurotoxicity (Rota rod test) of the ethanol extract was also determined. In vitro neuroprotective effect of the ethanol extract of Galium spurium was assessed by acetylcholinesterase and butrylcholinesterase inhibitions. Ultra Performance Liquid Chromatography-Time of Flight Mass Spectrometer (UPLC-TOF-MS) was used to identify the major compounds in the extract. In pentylenetetrazole-induced seizure, the ethanol extract at doses of 250 and 1000mg/kg prolonged the onset of seizures. Similarly, Galium spurium (250 and 500mg/kg) significantly delayed the onset of picrotoxin-induced seizures in mice and these doses also exhibited 12.5% and 17% protection, respectively, against picrotoxin-induced seizures. Furthermore, Galium spurium extract showed a...Continue Reading

References

Jul 1, 1993·Antimicrobial Agents and Chemotherapy·A De SarroG B De Sarro
Aug 15, 1998·Journal of Ethnopharmacology·G J AmabeokuJ A Syce
Aug 5, 1999·Journal of Ethnopharmacology·M H PourgholamiM Sayyah
Jun 14, 2000·Seizure : the Journal of the British Epilepsy Association·W M NsourI C Wong
Jun 8, 2001·Journal of Ethnopharmacology·E N BumP Herrling
Jan 29, 2002·Pharmacology, Biochemistry, and Behavior·G S SonavaneS B Kasture
May 2, 2002·Neuroscience·Claude Gottesmann
Jun 6, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·M SayyahM Kamalinejad
Jun 6, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·E BienvenuE P Springfield
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Jan 20, 2007·Phytotherapy Research : PTR·M F TavianoE M Galati
Feb 12, 2008·Progress in Neuro-psychopharmacology & Biological Psychiatry·Marjan Nassiri-AslFarzaneh Zamansoltani
Mar 17, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·I OrhanB Sener
Dec 23, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F S SenolB Sener
Jan 18, 2011·CNS Neuroscience & Therapeutics·Kenneth Rockwood
Mar 1, 2011·Journal of Ethnopharmacology·Neeraj ChoudharyAjudhia N Kalia
Jul 26, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jianming GuoYi You
Nov 11, 2011·CNS Neuroscience & Therapeutics·Daniel FriedmanNikolaos Scarmeas

❮ Previous
Next ❯

Citations

Jan 1, 2016·Journal of Chromatography. a·André de VilliersHarald Pasch
Jun 16, 2015·Epilepsy & Behavior : E&B·Nikolaus J Sucher, Maria C Carles
Sep 8, 2015·Journal of Pharmaceutical and Biomedical Analysis·Nassima ChaherTristan Richard
May 27, 2014·Progress in Neuro-psychopharmacology & Biological Psychiatry·Dorota NieoczymPiotr Wlaź
Apr 18, 2016·Journal of Ethnopharmacology·Harika AtmacaHafize Dilek Tepe
Feb 6, 2015·Oxidative Medicine and Cellular Longevity·Tâmara Coimbra DinizJackson Roberto Guedes da Silva Almeida
Mar 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gulay OzkanEsra Capanoglu
Feb 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dayanne Terra Tenório NonatoEdna Maria Camelo Chaves

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.