UPLC/MS based method for quantitative determination of fatty acid composition in Gram-negative and Gram-positive bacteria

Journal of Microbiological Methods
Merli SpitsmeisterRaivo Vilu

Abstract

Quantitative fatty acid composition of microorganisms at various growth space points is required for understanding membrane associated processes of cells, but the majority of the relevant publications still restrict to the relative compositions. In the current study, a simple and reliable method for quantitative measurement of fatty acid content in bacterial biomass without prior derivatization using ultra performance liquid chromatography-electrospray ionization mass spectrometry was developed. The method was applied for investigating the influence of specific growth rate and pH on the fatty acid profiles of two biotechnologically important microorganisms - Gram-negative bacteria Escherichia coli and Gram-positive bacteria Lactococcus lactis grown in controlled physiological states. It was found that the membranes of slowly growing cells are more rigid and that the fatty acid fraction of the cells of L. lactis diminishes considerably with increasing growth rate.

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Citations

Mar 16, 2011·Analytical Biochemistry·David G McLarenThomas P Roddy
Jan 28, 2012·Systematic and Applied Microbiology·Limin ZhangYing Huang
Aug 20, 2010·Applied Microbiology and Biotechnology·Igor G MinkevichTat'yana I Chistyakova

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