Upregulated 14‑3‑3β aggravates restenosis by promoting cell migration following vascular injury in diabetic rats with elevated levels of free fatty acids

International Journal of Molecular Medicine
Lishuai FengJingjing Liu

Abstract

Mono‑unsaturated free fatty acids (FFAs) can serve as a predictive indicator of vascular restenosis following interventional therapy, particularly in individuals with high‑fat diet‑induced type 2 diabetes. However, the pathogenic mechanism remains to be fully elucidated. In the present study, the levels of tyrosine 3‑monooxygenase/tryptophan 5‑monooxygenase activation protein β (YWHAB; also known as 14‑3‑3β), in vascular smooth muscle cells (VSMCs) treated with different concentrations of oleic acid (OA) were examined by reverse transcription‑quantitative polymerase chain reaction and western blot analyses. The migration of VSMCs was examined using wound‑healing and Transwell migration assays. The protein distribution of B‑cell lymphoma 2 (BCL‑2)‑associated death promoter (BAD) in VSMCs treated with OA was examined by immunofluorescence and western blot analyses. In in vivo experiments, the carotid artery morphology of rats in different groups was assessed at 14 days post‑injury by non-invasive ultrasonographic imaging and confirmed by histological staining. The expression of YWHAB was upregulated by OA in a concentration‑dependent manner in VSMCs. In the in vivo experiments, carotid stenosis was more serious among high‑FFA dia...Continue Reading

References

Feb 14, 2002·The Journal of Biological Chemistry·Fuminori TsurutaYukiko Gotoh
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 22, 2002·JAMA : the Journal of the American Medical Association·Joshua A BeckmanPeter Libby
Jun 23, 2004·Cytokine & Growth Factor Reviews·Elaine W Raines
Sep 8, 2004·Journal of Molecular and Cellular Cardiology·Erik Wilker, Michael B Yaffe
Dec 24, 2005·The New England Journal of Medicine·David M NathanUNKNOWN Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications (DCCT/EDIC) Study Research
Sep 3, 2010·Current Vascular Pharmacology·Danna M Breen, Adria Giacca
Sep 4, 2010·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Soon Jun HongDo-Sun Lim
Sep 12, 2012·Annals of Surgery·Diego M CastillaOmaida C Velazquez
Feb 16, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Takashi AshinoSatoshi Numazawa
Apr 23, 2013·Journal of Lipid Research·Christian BindesbøllKnut Tomas Dalen
Jul 31, 2013·Proteome Science·Yang ZhangYouhe Gao
Dec 24, 2013·BioMed Research International·Tadayuki ShimadaKanato Yamagata
Jul 30, 2014·Journal of Atherosclerosis and Thrombosis·Daisuke WajimaHiroyuki Nakase
Apr 26, 2015·Prostaglandins & Other Lipid Mediators·Ling-yun ChuKenneth K Wu
Aug 4, 2015·Journal of Diabetes and Its Complications·Shu-Chen KuoFung-Chung Sung
Sep 12, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hu QiJiang Hong
May 27, 2017·European Journal of Medicinal Chemistry·Alwin M Hartman, Anna K H Hirsch
Jun 21, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xu MaJianbo Wang
Sep 26, 2017·DNA and Cell Biology·Anbo GaoYuncheng Lv

❮ Previous
Next ❯

Citations

Dec 10, 2021·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Chaoxi ZhouHaixia Ding
Dec 7, 2021·Frontiers in Immunology·Samantha Rodríguez-MuguruzaMatilde R Chacón

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Carotid Artery Diseases

Carotid artery disease is a group of pathological conditions of the carotid artery. Discover the latest research on carotid artery disease here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.