Upregulated miR-1258 regulates cell cycle and inhibits cell proliferation by directly targeting E2F8 in CRC

Cell Proliferation
Zhiyuan ZhangYueming Sun

Abstract

MicroRNAs (miRNAs) as small noncoding RNA molecules function by regulating their target genes negatively. MiR-1258 was widely researched in multicancers, but its role remains unclear in colorectal cancer (CRC). The expression of miR-1258 and its specific target gene were detected in human CRC specimens and cell lines by miRNA RT-PCR, qRT-PCR and Western blot. The effects of miR-1258 on CRC proliferation were evaluated using CCK-8 assays, EdU incorporation, colony formation assays and cell-cycle assays; in vitro and the in vivo effects were investigated using a mouse tumorigenicity model. Luciferase reporter and RIP assays were employed to identify interactions between miR-1258 and its specific target gene. MiR-1258 was downregulated in CRC tissues and CRC cell lines, and upregulated miR-1258 was proved to inhibit proliferation and arrest cell cycle at G0/G1 in vitro and vivo. Luciferase reporter, RIP and western blot assays revealed E2F8 to be a direct target of miR-1258. The effects of miR-1258 in proliferation and cell cycle regulation can be abolished by E2F8 through rescue experiments. By directly targeting E2F8, miR-1258 influenced the expression of several cell-cycle factors, including cyclin D1 (CCND1) and cyclin depende...Continue Reading

References

Dec 16, 1993·Nature·Y XiongD Beach
May 18, 2001·Nature·G I Evan, K H Vousden
Nov 19, 2004·Nature·Joan Massagué
Sep 24, 2005·Nucleic Acids Research·Jesper ChristensenKristian Helin
Dec 17, 2009·Cancer Metastasis Reviews·Sylvia K Shenouda, Suresh K Alahari
Jan 27, 2011·Cancer Research·Lixin ZhangDario Marchetti
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Dec 3, 2011·Science Translational Medicine·Amin BahubeshiWilliam D Foulkes
Apr 11, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hongcheng LiuGening Jiang
Oct 29, 2013·Chemical Society Reviews·Jinbo LiYan Zhang
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
May 15, 2015·Nature·Herb Brody
Jun 29, 2016·Journal of Experimental & Clinical Cancer Research : CR·Laura PaladiniLibero Santarpia
Mar 9, 2017·Journal of Experimental & Clinical Cancer Research : CR·Jing SunChuan Su

❮ Previous
Next ❯

Citations

Jul 19, 2019·Experimental and Therapeutic Medicine·Jie XuJianping Shao
Sep 8, 2019·Journal of Experimental & Clinical Cancer Research : CR·Wen PengYueming Sun
Apr 23, 2019·Journal of Oncology·Duncan AyersManuel Macías-González
Nov 7, 2019·Journal of Cellular Biochemistry·Xuemei JiFangyu Wang
Jun 24, 2019·Journal of Cellular Physiology·Dongjian JiYueming Sun
Sep 10, 2019·Cell Biology International·Taotao QianYong Zhu
Dec 12, 2020·Frontiers in Cell and Developmental Biology·Zhiyuan ZhangGuodong He
Dec 19, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ning ZhangJuan Du
Jan 25, 2021·Journal of Experimental & Clinical Cancer Research : CR·Fangyu ChenXinchen Sun
Nov 3, 2020·Clinical and Translational Medicine·Peng YangYueming Sun
Feb 10, 2019·Biochemical and Biophysical Research Communications·Wei LiuWeihua Cai
Jun 29, 2021·Frontiers in Oncology·Lucas Maciel VieiraMaria Emília Machado Telles Walter
Jul 20, 2021·Cancer Management and Research·ZhaoHui XuXin Chen
Jul 31, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ke-Da YangZhi-Jun Zeng
Aug 7, 2021·Archives of Physiology and Biochemistry·Li ZhengYan Li
Aug 29, 2021·Journal of Experimental & Clinical Cancer Research : CR·Chaofan PengYueming Sun
Aug 28, 2021·Genes·Yu ShiChang Gong
Jan 23, 2022·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·J TanY Zhu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.