Upregulated miR-221/222 promotes cell proliferation and invasion and is associated with invasive features in retinoblastoma

Cancer Biomarkers : Section a of Disease Markers
Hongqian LiuXinfeng Liu

Abstract

MicroRNA (miR-221/222) is frequently overexpressed in many cancers and is associated with poor prognosis. However, the role of miR-221/222 in retinoblastoma (RB) remains unclear. This study aimed to detect the clinical significance of miR-221/222 in RB patients and explore its role in RB cells in vitro. Expression of miR-221/222 was assessed in fresh RB tissue collected from 64 eyes and normal retinal tissues from 18 unrelated donor cadaver eyes by quantitative real time RT-PCR analysis (qRT-PCR), and correlated with the histopathological findings. Human RB Y79 cells were transfected with miR-221/222 precursors or inhibitors to overexpress or downregulate miR-221/222 expression, respectively, using Lipofectamine 2000 reagent. The biological effects of miR-221/222 were then assessed by cell viability assays, colony formation assays, apoptosis detection assays, Matrigel® invasion assays, and wound-healing assays. Higher miR-221/222 expression was detected in RB tissues compared to that of the normal retinal tissues (p< 0.001). Higher miR-221/222 expression was correlated with invasion in patients with RB. Targeting of miR-221/222 induced apoptosis and inhibited Y79 cell proliferation, migration, and invasion in vitro. However, ov...Continue Reading

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Citations

Aug 23, 2019·Wiley Interdisciplinary Reviews. RNA·Shama Prasada KabekkoduKapaettu Satyamoorthy
Oct 24, 2018·Journal of Cancer Research and Clinical Oncology·Lara Elis Alberici DelsinMaria Sol Brassesco
Jan 14, 2020·Journal of Oncology·Qian SongXin Cao
Jul 3, 2021·International Journal of Molecular Sciences·Adriana FodorAngela Cozma

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