Upregulation and biological function of transmembrane protein 119 in osteosarcoma

Experimental & Molecular Medicine
Zhen-Huan JiangZhi-Jun Ge

Abstract

Osteosarcoma is suggested to be caused by genetic and molecular alterations that disrupt osteoblast differentiation. Recent studies have reported that transmembrane protein 119 (TMEM119) contributes to osteoblast differentiation and bone development. However, the level of TMEM119 expression and its roles in osteosarcoma have not yet been elucidated. In the present study, TMEM119 mRNA and protein expression was found to be up-regulated in osteosarcoma compared with normal bone cyst tissues. The level of TMEM119 protein expression was strongly associated with tumor size, clinical stage, distant metastasis and overall survival time. Moreover, gene set enrichment analysis (GSEA) of the Gene Expression Omnibus (GEO) GSE42352 dataset revealed TMEM119 expression in osteosarcoma tissues to be positively correlated with cell cycle, apoptosis, metastasis and TGF-β signaling. We then knocked down TMEM119 expression in U2OS and MG63 cells using small interfering RNA, which revealed that downregulation of TMEM119 could inhibit the proliferation of osteosarcoma cells by inducing cell cycle arrest in G0/G1 phase and apoptosis. We also found that TMEM119 knockdown significantly inhibited cell migration and invasion, and decreased the expressio...Continue Reading

References

Jul 1, 1987·Endocrinology·J PfeilschifterG R Mundy
Aug 15, 1997·The Biochemical Journal·G M Cohen
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Apr 7, 2006·Journal of Cellular Biochemistry·David Thomas, Maya Kansara
Nov 1, 2006·Clinical Orthopaedics and Related Research·Rex C HaydonTong-Chuan He
Jun 10, 2008·Developmental Cell·Jing Yang, Robert A Weinberg
Dec 23, 2008·Cancer Biology & Therapy·Mei Lin TanCrispin R Dass
Mar 4, 2009·The Journal of Bone and Joint Surgery. American Volume·Theresa A GuiseRichard M Terek
Sep 25, 2009·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·P DoolanL O'Driscoll
Feb 6, 2010·Expert Review of Anticancer Therapy·Sameer Bakhshi, Venkatraman Radhakrishnan
Mar 10, 2010·Cancer Treatment and Research·Giulia Ottaviani, Norman Jaffe
Mar 16, 2012·Development, Growth & Differentiation·Koji MizuhashiTakahisa Furukawa
Sep 18, 2012·Pharmacology & Therapeutics·Jonathan GillRichard Gorlick
May 24, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guanzhong QiuGuohan Hu
Jul 23, 2015·Journal of Hematology & Oncology·Bin LiShi-Ting Li
Aug 5, 2015·Journal of Experimental & Clinical Cancer Research : CR·Zhongping ChengXiaoyan Qu
Sep 24, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wenliang QiaoQiang Lin

❮ Previous
Next ❯

Citations

Dec 20, 2019·Translational Vision Science & Technology·Nan SuPeter Heiduschka
Jan 14, 2021·Cell Death Discovery·Daohu PengLi Sen
Aug 12, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Butian ZhangJinlan Jiang
Jun 27, 2021·Digestive Diseases and Sciences·Kai Nie, Mingquan Cai

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE42352
GSE39055
and

Methods Mentioned

BETA
PCR
transfection
flow cytometry
xenografts
xenograft

Software Mentioned

GraphPad PRISM
ImageJ
GSEA

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.