PMID: 11909638Mar 23, 2002Paper

Upregulation by retinoic acid of transforming growth factor-beta-stimulated heat shock protein 27 induction in osteoblasts: involvement of mitogen-activated protein kinases

Biochimica Et Biophysica Acta
D HatakeyamaToshihiko Uematsu

Abstract

We investigated whether transforming growth factor-beta (TGF-beta) stimulates the induction of heat shock protein (HSP) 27 and HSP70 in osteoblast-like MC3T3-E1 cells and the mechanism underlying the induction. TGF-beta increased the level of HSP27 but had no effect on the HSP70 level. TGF-beta stimulated the accumulation of HSP27 dose-dependently, and induced an increase in the level of mRNA for HSP27. TGF-beta induced the phosphorylation of p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase. The HSP27 accumulation induced by TGF-beta was significantly suppressed by PD98059, an inhibitor of the upstream kinase of p44/p42 MAP kinase, or SB203580, an inhibitor of p38 MAP kinase. PD98059 and SB203580 suppressed the TGF-beta-stimulated increase in the level of mRNA for HSP27. Retinoic acid, a vitamin A (retinol) metabolite, which alone had little effect on the HSP27 level, markedly enhanced the HSP27 accumulation stimulated by TGF-beta. Retinoic acid enhanced the TGF-beta-induced increase of mRNA for HSP27. The amplification of TGF-beta-stimulated HSP27 accumulation by retinoic acid was reduced by PD98059 or SB203580. Retinoic acid failed to affect the TGF-beta-induced phosphorylation of p44/p42 MAP kinase or p38 MA...Continue Reading

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Citations

Nov 26, 2010·Cell Stress & Chaperones·Hossein PourghadamyariGordon A Ferns
Nov 26, 2011·Cell Stress & Chaperones·Eunna Chung, Marissa Nichole Rylander
Jun 26, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Regina VarghaChristoph Aufricht
Jun 22, 2012·American Journal of Physiology. Renal Physiology·Hong-bo XiaoLin Sun
Feb 28, 2004·Seminars in Cell & Developmental Biology·Thomas H MacRae
Jul 12, 2016·Molecular and Cellular Endocrinology·Naohiro YamamotoTakanobu Otsuka
Jun 13, 2003·Archives of Biochemistry and Biophysics·Haruhiko TokudaOsamu Kozawa
Feb 10, 2004·Molecular & Cellular Proteomics : MCP·Johan MalmströmGyörgy Marko-Varga
Aug 18, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Boon Chin HengBjorn Olsen
Sep 7, 2018·Cell Stress & Chaperones·Kai HangZhijun Pan
Feb 15, 2019·The Journal of Reproduction and Development·Islam M SaadeldinAbdullah N Alowaimer
Sep 14, 2017·International Journal of Molecular Medicine·Shingo KainumaTakanobu Otsuka
Aug 1, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Shinji TakaiOsamu Kozawa

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