Upregulation of 78-kDa glucose-regulated protein in macrophages in peripheral joints of active ankylosing spondylitis

Scandinavian Journal of Rheumatology
W DongP Zhu

Abstract

To investigate the expression of the 78-kDa glucose-regulated protein (GRP78), a marker of activation of the unfolded protein response (UPR), in macrophages of ankylosing spondylitis (AS) synovium and synovial fluid and to explore the relationship between the expression of GRP78 and synovial fluid levels of the proinflammatory cytokines, as well as the inflammatory activity of AS. Immunohistochemical staining and Western blotting were performed to detect the expression of GRP78 in macrophages in the involved peripheral joints of 16 patients with active AS, and in 15 patients with osteoarthritis (OA) as controls. Synovial fluid levels of tumour necrosis factor-alpha (TNFalpha) and interleukin-6 (IL-6) of AS peripheral joints were measured using an enzyme-linked immunosorbent assay (ELISA). The inflammatory activity of AS was measured by the sera levels of IL-6 and the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Expression of GRP78 in macrophages was increased significantly in the affected peripheral joints of AS compared with OA patients. Positive correlations were found between the expression of GRP78 in macrophages in AS-affected peripheral joints and the synovial fluid levels of TNFalpha and IL-6, as well as ...Continue Reading

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Citations

Nov 26, 2008·Toxicology and Applied Pharmacology·Gisele FariaMarcos A Rossi
Mar 3, 2010·Immunological Reviews·Robert A ColbertGerlinde Layh-Schmitt
Mar 27, 2016·Brain Research·Pierre-Jean Le ResteEric Chevet
Nov 17, 2015·Clinical Rheumatology·Judith A Smith
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Apr 12, 2015·Annual Review of Immunology·Paul Bowness
Jul 1, 2016·Brain Research·Pierre-Jean Le ResteEric Chevet
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May 17, 2019·Nature Reviews. Rheumatology·Robert BuschElizabeth D Mellins
Jun 2, 2018·Rheumatology International·José Pablo Romero-LópezEthel García-Latorre

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