Upregulation of a tonoplast-localized cytochrome P450 during petal senescence in Petunia inflata.

BMC Plant Biology
Yan XuM R Hanson

Abstract

Gene expression in Petunia inflata petals undergoes major changes following compatible pollination. Severe flower wilting occurs reproducibly within 36 hours, providing an excellent model for investigation of petal senescence and programmed cell death. Expression of a number of genes and various enzyme activities involved in the degradation and remobilization of macromolecules have been found to be upregulated during the early stages of petal senescence. By performing differential display of cDNAs during Petunia inflata petal senescence, a highly upregulated gene encoding a cytochrome P450 was identified. Analysis of the complete cDNA sequence revealed that the predicted protein is a member of the CYP74C family (CYP74C9) and is highly similar to a tomato CYP74C allene oxide synthase (AOS) that is known to be active on 9-hydroperoxides. Cloning of the petunia genomic DNA revealed an intronless gene with a promoter region that carries signals found in stress-responsive genes and potential binding sites for Myb transcription factors. Transcripts were present at detectable levels in root and stem, but were 40 times more abundant in flowers 36 hours after pollination. Ethylene and jasmonate treatment resulted in transitory increases...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C B TaylorP J Green
Feb 1, 1997·Trends in Genetics : TIG·C Martin, J Paz-Ares
Feb 10, 1999·Molecular Biotechnology·P Liang, A B Pardee
Aug 27, 1999·Archives of Biochemistry and Biophysics·D R Nelson
Sep 11, 1999·Plant Physiology·J C Stein, G Hansen
Apr 1, 2000·The Plant Journal : for Cell and Molecular Biology·H MaucherC Wasternack
Nov 4, 2000·The Journal of Biological Chemistry·A Itoh, G A Howe
Feb 24, 2001·Plant Molecular Biology·B Rubinstein
Oct 20, 2001·Current Opinion in Plant Biology·E P Beers, J M McDowell
Feb 15, 2002·The Plant Journal : for Cell and Molecular Biology·Marc J Curtis, Thomas J Wolpert
Apr 19, 2002·Current Opinion in Plant Biology·Gregg A Howe, Anthony L Schilmiller
Jun 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomohiro UemuraMasa H Sato
Aug 3, 2002·Plant & Cell Physiology·Youichiro FukaoMikio Nishimura
Sep 12, 2002·Annual Review of Plant Biology·Ivo Feussner, Claus Wasternack
Jan 1, 1994·The Plant Cell·C. A. Conley, M. R. Hanson
Mar 15, 2003·The Journal of Biological Chemistry·Moonil KimHyun-Sook Pai
Sep 25, 2003·Annual Review of Plant Biology·Mary A Schuler, Daniele Werck-Reichhart
Nov 18, 2003·Annual Review of Genetics·Zachary NimchukJeffery L Dangl
Jun 1, 1995·Trends in Cell Biology·W C Earnshaw
Feb 3, 2004·Journal of Experimental Botany·Ernest Y Kwok, Maureen R Hanson

❮ Previous
Next ❯

Citations

Mar 4, 2008·Journal of Experimental Botany·Wouter G van Doorn, Ernst J Woltering
Nov 28, 2017·Plant Biology·N WojciechowskaA Bagniewska-Zadworna
Jan 1, 2013·Antioxidants·Marina CavaiuoloAntonio Ferrante
Oct 25, 2007·Chembiochem : a European Journal of Chemical Biology·Alexander N GrechkinLucia S Mukhtarova
Nov 1, 2007·Plant Signaling & Behavior·Siddharth Kaushal Tripathi, Narendra Tuteja
Oct 18, 2019·Plant Molecular Biology·Mst Hur MadinaHugo Germain
Sep 10, 2008·Chembiochem : a European Journal of Chemical Biology·Alexander N GrechkinMats Hamberg

❮ Previous
Next ❯

Datasets Mentioned

BETA
DQ351288
AF454634
AF081955
AJ271093
AF317515
AF230372
AF087932
AY242385
AB077765
AY514020

Methods Mentioned

BETA
transgenic
fluorescence
electrophoresis
PCR
cDNA library
Protein Assay

Software Mentioned

Predotar
PAUP
Psort
TargetP
GenHunter

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis