PMID: 8938099Dec 1, 1996Paper

Upregulation of adhesion molecule expression on endothelial cells by anti-DNA autoantibodies in systemic lupus erythematosus

Clinical Immunology and Immunopathology
K N LaiC K Lai

Abstract

The mechanism of vasculopathy in systemic lupus erythematosus (SLE) remains unclear and the evidence for a direct pathogenic role of anti-double-stranded DNA antibodies (anti-dsDNA) is not strong. Our study aims to determine whether anti-dsDNA exerts any effect on the expression of adhesion molecules on endothelial cells. IgG was purified from 17 patients with SLE (median anti-dsDNA titer, 404 IU/ml) and from 9 healthy controls (median titer 16 IU/ml). Anti-dsDNA-depleted polyclonal IgG (anti-dsDNA-dep-IgG) (median anti-dsDNA titer 17 IU/ml) was prepared from sera of these patients with SLE by affinity chromatography with DNA cellulose column. Expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin on human umbilical vein endothelial cells (HUVEC) cultured with either control IgG or anti-dsDNA were compared by flow cytometry. The levels of adhesion molecules in the supernatant of cultured HUVEC were assessed by sandwich ELISA. Compared with either control IgG or anti-dsDNA-dep-IgG, HUVEC incubated with anti-dsDNA expressed a significantly higher mean fluorescence intensity of ICAM-1 and in VCAM-1 and a higher supernatant concentration of ICAM-1 and VCAM-1 but not E-se...Continue Reading

Citations

Jun 23, 1999·The American Journal of Medicine·C G Kevil, D C Bullard
May 10, 2005·Nephrology·Sydney TangKar Neng Lai
Nov 17, 2009·[Rinshō ketsueki] The Japanese journal of clinical hematology·Tatsuya Atsumi
Jun 1, 2005·Rheumatic Diseases Clinics of North America·Andrea DoriaYehuda Shoenfeld
Feb 13, 2008·Clinical and Experimental Nephrology·Mizue IshikawaIsao Kubota
Dec 6, 2005·Immunobiology·Pierre Youinou
Oct 30, 2015·Journal of Clinical Pathology·V SalleJ P Ducroix
Mar 22, 2015·Seminars in Nephrology·Sudhanshu Kumar Verma, Bruce A Molitoris
Nov 1, 2007·Expert Review of Clinical Immunology·Mohammed Yousuf KarimDavid P D'Cruz
Feb 8, 2008·American Journal of Physiology. Renal Physiology·Kar Neng LaiSydney C W Tang
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mileka R GilbertBarbara J Vilen
Jan 1, 2014·International Scholarly Research Notices·Hasni MahayidinWan Zuraida Wan Ab Hamid

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