Upregulation of amyloid precursor protein and its mRNA in an experimental model of paediatric head injury

Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia
Van Den Heuvel CR A Pereira

Abstract

Amyloid precursor protein (APP), a membrane spanning glycoprotein which plays an important role in neuronal growth and synaptic plasticity, is increased after traumatic brain injury (TBI) and has been used as a sensitive marker of neuronal damage in an adult sheep head impact model. We hypothesised that APP expression would similarly be increased in lambs, suggesting that in the immature injured brain APP is also upregulated as an acute phase response to trauma. Ten anaesthetised and ventilated 4-5 week old Merino lambs sustained a left temporal head impact from a humane stunner. At 2 h after impact, there was widespread and intense neuronal cell body APP immunoreactivity which was more widely distributed than axonal APP. APP messenger RNA (mRNA) expression was also markedly increased with a distribution similar to that of APP antigen. These results demonstrate that APP antigen and mRNA are sensitive early indicators of TBI in paediatric cases.

Citations

Aug 26, 2006·Molecular Neurodegeneration·Hui Zheng, Edward H Koo
Apr 30, 2011·Molecular Neurodegeneration·Hui Zheng, Edward H Koo
Aug 16, 2002·Australian Veterinary Journal·J W FinnieG E Summersides
Jan 8, 2015·Frontiers in Neurology·Gregory A ElderStephen Ahlers
Jun 17, 2005·Journal of Neural Transmission·J SzczygielskiO Wirths
Oct 5, 2017·Neurology. Genetics·Jessica GillAngela Yarnell
Nov 27, 2002·Veterinary Pathology·J W Finnie, P C Blumbergs

Related Concepts

Brain
Cell Density
Temporal Region Trauma
Immunocytochemistry
Ambidexterity
Neurons
Poly(A) Tail
Dall Sheep
Receptor Up-Regulation
Amyloid beta-Protein Precursor

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