Upregulation of basal TGFbeta1 levels by EMF coincident with chondrogenesis--implications for skeletal repair and tissue engineering

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
R K AaronD M Ciombor

Abstract

Members of the TGFbeta/BMP gene family regulate cartilage and bone development. These genes are re-expressed in bone repair and are thought to mediate chondro- and osteoprogenitor cell differentiation. These observations have led to a therapeutic strategy of introducing these growth factors into experimental cartilage and bone defects. Therapeutic efficacy, however, has been limited by diffusion or inactivation of these growth factors from the desired site and by the inability to deliver sustained concentrations of growth factors. This study demonstrates an increase in basal TGFbeta mRNA and protein levels in association with chondrogenic differentiation in endochondral ossification. mRNA is increased by 158%; protein by 23%, and cells immunopositive for TGFbeta by 343% at maximal TGFbeta expression. Importantly, the pattern of TGFbeta expression is preserved throughout the developmental sequence. Our data suggest that the exposure to a specific electromagnetic field (EMF) enhances, but does not disorganize, chondrogenesis and endochondral calcification as well as the normal physiologic expression of TGFbeta. The ability to increase TGFbeta at a moderately low dose for sustained periods of time without disorganizing its physiol...Continue Reading

References

Jul 1, 1992·The Journal of Clinical Investigation·W A Border, E Ruoslahti
May 1, 1990·The Journal of Bone and Joint Surgery. British Volume·W J Sharrard
Aug 9, 1990·Journal of Theoretical Biology·K J McLeod, C T Rubin
Oct 1, 1988·Analytical Biochemistry·Y J KimA J Grodzinsky
Oct 1, 1988·Calcified Tissue International·R K AaronH J Barrach
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A H Reddi, C Huggins
Sep 1, 1983·Calcified Tissue International·D P DeSimone, A H Reddi
May 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M P BostromV M Rosen
May 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R J FitzsimmonsD J Baylink
Feb 1, 1994·Acta Orthopaedica Scandinavica·H M NielsenH Oxlund
Oct 1, 1993·Acta Orthopaedica Scandinavica·M LindC Bünger
Jan 1, 1993·International Review of Experimental Pathology·T G TerrellJ D Green
Nov 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y IshidouT Sakou
Jul 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R K Aaron, D M Ciombor
Jan 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T MaruiS L Woo
Jan 26, 1999·Clinical Orthopaedics and Related Research·A E GoodshipJ Kenwright
Oct 29, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C H LohmannB D Boyan
Mar 16, 2001·Clinical Orthopaedics and Related Research·H H GuerkovB D Boyan
Feb 21, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Deborah McK CiomborBruce Caterson

❮ Previous
Next ❯

Citations

Mar 3, 2007·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·C ZorziS Setti
Mar 27, 2010·International Orthopaedics·P R J V C BoopalanSamuel Chittaranjan
Apr 5, 2008·Rheumatology International·Bernhard Schmidt-RohlfingKarsten Gavenis
Nov 11, 2010·Archives of Orthopaedic and Trauma Surgery·Hendrik JansenRainer H Meffert
Apr 11, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Jean C Gan, Paul A Glazer
Jul 3, 2007·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Jack B HaddadPhillip Shinnick
Aug 10, 2012·Tissue Engineering. Part B, Reviews·Richard BalintSarah H Cartmell
Oct 1, 2011·BMC Musculoskeletal Disorders·Shuai DingZhe Wang
Nov 28, 2006·Head & Face Medicine·Edela PuricelliJoão Julio da Cunha Filho
Jun 1, 2013·Tissue Engineering. Part C, Methods·Mariea A BradyC Ross Ethier
Jan 18, 2012·International Orthopaedics·Olav P van der JagtHarrie Weinans
Dec 3, 2014·Clinics in Podiatric Medicine and Surgery·Jeremy J CookEmily A Cook
Nov 22, 2005·Foot and Ankle Clinics·Deborah McK Ciombor, Roy K Aaron
Jul 13, 2006·Annals of the New York Academy of Sciences·Roy K AaronBruce Simon
Oct 18, 2013·Electromagnetic Biology and Medicine·Hamed Reihani KermaniSaeed Esmaeili Mahani
Jul 13, 2014·Bone & Joint Research·O P van der JagtH Weinans
Mar 19, 2016·Journal of Biomedical Materials Research. Part a·Hee-Gyeong YiDong-Woo Cho
Jan 27, 2009·Progress in Histochemistry and Cytochemistry·Richard H W FunkNurdan Ozkucur
Jan 22, 2008·Clinics in Sports Medicine·Norbert BachlHarald Tschan
Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·C MothersillC B Seymour
Apr 4, 2013·Dose-response : a Publication of International Hormesis Society·Carmel MothersillRaimond Wong
May 16, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nagarajan SelvamuruganNicola C Partridge
Jul 17, 2007·Plastic and Reconstructive Surgery·Berish StrauchArthur A Pilla
Jul 19, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M FiniR Giardino
Jan 8, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Franco BenazzoRoberto Giardino
Sep 15, 2005·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Hyun J PaekMichael J Lysaght
Apr 26, 2008·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Nuray EminY Murat Elçin
May 23, 2014·Scandinavian Journal of Medicine & Science in Sports·J HuH Lu
Jun 23, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Oren M TepperGeoffrey C Gurtner
Feb 7, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·F VeronesiM Fini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.