Upregulation of BMSCs osteogenesis by positively-charged tertiary amines on polymeric implants via charge/iNOS signaling pathway

Scientific Reports
Wei ZhangPaul K Chu

Abstract

Positively-charged surfaces on implants have a similar potential to upregulate osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) as electromagnetic therapy approved for bone regeneration. Generally, their osteogenesis functions are generally considered to stem from the charge-induced adhesion of extracellular matrix (ECM) proteins without exploring the underlying surface charge/cell signaling molecule pathways. Herein, a positively-charged surface with controllable tertiary amines is produced on a polymer implant by plasma surface modification. In addition to inhibiting the TNF-α expression, the positively-charged surface with tertiary amines exhibits excellent cytocompatibility as well as remarkably upregulated osteogenesis-related gene/protein expressions and calcification of the contacted BMSCs. Stimulated by the charged surface, these BMSCs display high iNOS expressions among the three NOS isoforms. Meanwhile, downregulation of the iNOS by L-Can or siRNA inhibit osteogenic differentiation in the BMSCs. These findings suggest that a positively-charged surface with tertiary amines induces osteogenesis of BMSCs via the surface charge/iNOS signaling pathway in addition to elevated ECM protein adhesion. Therefor...Continue Reading

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Citations

Feb 19, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Saber GhadakzadehMaryam Tabrizian
Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Alieh AminianKurosch Rezwan
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Jul 2, 2018·Journal of Materials Science. Materials in Medicine·André SchulzHeiko Zimmermann
Apr 29, 2021·Biointerphases·Ahmet Engin PazarçevirenZafer Evis
Jun 25, 2021·Advanced Science·Donghui WangXuanyong Liu
Aug 8, 2021·International Journal of Molecular Sciences·Chien-Ching LeeMon-Juan Lee
Sep 9, 2019·ACS Biomaterials Science & Engineering·Zhengnan ZhouGuoxin Tan
Aug 28, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Fuqiang LiuPaul K Chu

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Methods Mentioned

BETA
atomic force microscopy
transfection
X-ray
flow cytometry
protein assay

Software Mentioned

Quantity One
Axio Observer
Multipak
FloMax
SPSS
LSM

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